Menu utilisateur

Accès à distance ? S'identifier sur le proxy UCLouvain

Control of trophectoderm differentiation by inner cell mass-derived fibroblast growth factor-4 in mouse blastocysts and corrective effect of FGF-4 on high glucose-induced trophoblast disruption.

Onglets principaux

Leunda-Casi, Amaya [UCL] De Hertogh, René [UCL] Pampfer, Serge [UCL]

Previous studies have suggested that fibroblast growth factor-4 (FGF-4) may be a paracrine signal used by inner cell mass (ICM) cells to maintain adjacent trophectoderm (TE) cells in an undifferentiated state. In the present work, immunocytochemical analysis of mouse blastocysts confirmed that FGF-4 was predominantly detected in the ICM before and after spreading over a fibronectin-coated culture substrate. Addition of human recombinant FGF-4 did not influence morphological progression, cell allocation and proliferation in ICM and TE lineages or mitosis and karyorhexis frequencies during blastocyst expansion. Addition of FGF-4 to outgrowing blastocysts, in contrast, induced a significant decrease in the surface of the trophoblast outgrowths formed by the TE cells and in the proportion of giant trophoblasts per outgrowth. The fact that blastocysts display excessive trophoblast expansion and spreading over their culture substrate upon pre-exposure to high concentrations of glucose in vitro was used to further assess the regulatory effect of FGF-4. Addition of FGF-4 was indeed found to fully neutralize the disruptive impact of high glucose on trophoblast outgrowths. Altogether, our data indicate that ICM-derived FGF-4 participates actively in the regulation of trophoblast development.

Type de document Article de périodique (Journal article) – Article de recherche
Type d'accès Accès restreint
Année de publication 2001
Information sur le périodique "Molecular Reproduction and Development" - Vol. 60, no. 1, p. 38-46 (2001)
Peer reviewed oui
Editeur JohnWiley & Sons, Inc. (Hoboken)
issn 1040-452X
e-issn 1098-2795
Statut de la publication Publié
Affiliations UCL - Autre
MESH Subject Animals ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cell Nucleus - drug effects ; Cell Size - drug effects ; Female ; Fibroblast Growth Factor 4 ; Fibroblast Growth Factors - metabolism - pharmacology ; Glucose - metabolism - pharmacology ; Humans ; Immunohistochemistry ; Male ; Mice ; Proto-Oncogene Proteins - metabolism - pharmacology ; Recombinant Proteins - pharmacology ; Trophoblasts - cytology - drug effects - metabolism
Mots-clés Outgrowth ; Giant trophoblast ; Immunocytochemistry ; Mitosis ; Karyorhexis
Liens
  1. Ansell, J Embryol Exp Morphol, 33, 177 (1975)
  2. Arman E., Haffner-Krausz R., Chen Y., Heath J. K., Lonai P., Targeted disruption of fibroblast growth factor (FGF) receptor 2 suggests a role for FGF signaling in pregastrulation mammalian development, 10.1073/pnas.95.9.5082
  3. Barlow, J Embryol Exp Morphol, 27, 447 (1972)
  4. Bhatnagar, Development, 121, 1333 (1995)
  5. Brison Daniel R., Schultz Richard M., RT-PCR-based method to localize the spatial expression of genes in the mouse blastocyst, 10.1002/(sici)1098-2795(199606)44:2<171::aid-mrd5>3.0.co;2-k
  6. Brison Daniel R., Schultz Richard M., Increased Incidence of Apoptosis in Transforming Growth Factor α-Deficient Mouse Blastocysts1, 10.1095/biolreprod59.1.136
  7. Chai Ning, Patel Yogesh, Jacobson Kristine, McMahon Jill, McMahon Andrew, Rappolee Daniel A., FGF Is an Essential Regulator of the Fifth Cell Division in Preimplantation Mouse Embryos, 10.1006/dbio.1997.8858
  8. Feldman B., Poueymirou W., Papaioannou V., DeChiara T., Goldfarb M., Requirement of FGF-4 for postimplantation mouse development, 10.1126/science.7809630
  9. Gardner, J Cell Sci, 10, 11 (1988)
  10. Haffner-Krausz Rebecca, Gorivodsky Marat, Chen Yali, Lonai Peter, Expression of Fgfr2 in the early mouse embryo indicates its involvement in preimplantation development, 10.1016/s0925-4773(99)00082-9
  11. Haimovici Florina, Anderson Deborah J., Effects of Growth Factors and Growth Factor-Extracellular Matrix Interactions on Mouse Trophoblast Outgrowth in Vitro1, 10.1095/biolreprod49.1.124
  12. Harvey Mark B., Kaye Peter L., Insulin-like growth factor-1 stimulates growth of mouse preimplantation embryos in vitro, 10.1002/mrd.1080310306
  13. Kanai-Azuma Masami, Kanai Yoshiakira, Kurohmaru Masamichi, Sakai Senkichi, Hayashi Yoshihiro, Insulin-Like Growth Factor (IGF)-I Stimulates Proliferation and Migration of Mouse Ectoplacental Cone Cells, While IGF-II Transforms them into Trophoblastic Giant Cells in Vitro1, 10.1095/biolreprod48.2.252
  14. Lea R. G., McCracken J. E., McIntyre S. S., Smith W., Baird J. D., Disturbed Development of the Preimplantation Embryo in the Insulin-Dependent Diabetic BB/E Rat, 10.2337/diab.45.11.1463
  15. Maro, J Embryol Exp Morphol, 81, 211 (1984)
  16. Moley Kelle, Diabetes and Preimplantation Events of Embryogenesis, 10.1055/s-2007-1016221
  17. Nichols Jennifer, Zevnik Branko, Anastassiadis Konstantinos, Niwa Hitoshi, Klewe-Nebenius Daniela, Chambers Ian, Schöler Hans, Smith Austin, Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4, 10.1016/s0092-8674(00)81769-9
  18. Niswander, Development, 114, 755 (1992)
  19. Pampfer S., Wuu Y. D., Vanderheyden I., De Hertogh R., In vitro study of the carry-over effect associated with early diabetic embryopathy in the rat, 10.1007/bf00400939
  20. Pampfer S, Wuu Y D, Vanderheyden I, De Hertogh R, Expression of tumor necrosis factor-alpha (TNF alpha) receptors and selective effect of TNF alpha on the inner cell mass in mouse blastocysts., 10.1210/endo.134.1.8275935
  21. Pampfer, Development, 124, 4827 (1997)
  22. Pampfer, J Reprod Fert, 55, 129 (2000)
  23. Patel Yogesh, Kim Heidi, Rappolee Daniel A., A Role for Hepatocyte Growth Factor During Early Postimplantation Growth of the Placental Lineage in Mice1, 10.1095/biolreprod62.4.904
  24. Rappolee, Development, 120, 2259 (1994)
  25. Rappolee Daniel A., Patel Yogesh, Jacobson Kristine, Expression of fibroblast growth factor receptors in peri-implantation mouse embryos, 10.1002/(sici)1098-2795(199811)51:3<254::aid-mrd4>3.0.co;2-o
  26. Shen M. M., Leder P., Leukemia inhibitory factor is expressed by the preimplantation uterus and selectively blocks primitive ectoderm formation in vitro., 10.1073/pnas.89.17.8240
  27. Tanaka S., Promotion of Trophoblast Stem Cell Proliferation by FGF4, 10.1126/science.282.5396.2072
  28. Taniguchi Fuminori, Harada Tasuku, Yoshida Souichi, Iwabe Tomio, Onohara Yoshimasa, Tanikawa Masahiro, Terakawa Naoki, Paracrine effects of bFGF and KGF on the process of mouse blastocyst implantation, 10.1002/(sici)1098-2795(199805)50:1<54::aid-mrd7>3.0.co;2-w
  29. Wuu Yan-Der, Pampfer Serge, Becquet Philippe, Vanderheyden Ivo, Lee Kun-Hsiung, De Hertogh René, Tumor Necrosis Factor α Decreases the Viability of Mouse Blastocysts In Vitro and In Vivo1, 10.1095/biolreprod60.2.479
Référence bibliographique Leunda-Casi, Amaya ; De Hertogh, René ; Pampfer, Serge. Control of trophectoderm differentiation by inner cell mass-derived fibroblast growth factor-4 in mouse blastocysts and corrective effect of FGF-4 on high glucose-induced trophoblast disruption.. In: Molecular Reproduction and Development, Vol. 60, no. 1, p. 38-46 (2001)
Permalien http://hdl.handle.net/2078.1/8941