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Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

Onglets principaux

Masereel, Bernard [UNamur] Dinguizli, Mustapha [FUNDP] Bouzin, Caroline [UCL] Moniotte, Nicolas [FUNDP] Feron, Olivier [UCL] Gallez, Bernard [UCL] Vander Borght, Thierry [UCL] Michiels , Carine [FUNDP] Lucas, Stéphane [FUNDP]

Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis.

Type de document Article de périodique (Journal article) – Article de recherche
Type d'accès Accès restreint
Année de publication 2011
Langue Anglais
Information sur le périodique "Journal of Nanoparticle Research : an interdisciplinary forum for nanoscale science and technology" - Vol. 13, no. 4, p. 1573-1580 (2011)
Peer reviewed oui
Editeur Springer Netherlands ((Netherlands) Dordrecht)
issn 1388-0764
e-issn 1572-896X
Statut de la publication Publié
Affiliations FUNDP - MED_Département de Pharmacie
UCL - SSS/LDRI - Louvain Drug Research Institute
FUNDP - Autre
UCL - SSS/IREC - Institut de recherche expérimentale et clinique
UCL - SSS/IREC/FATH - Pôle de Pharmacologie et thérapeutique
UCL - (MGD) Service de médecine nucléaire
Mots-clés Gold ; Nanoparticles ; Polymer ; Antibody ; Polyallylamine ; Polystyrenesulfonate ; Nanomedicine ; Drug delivery
Liens
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Référence bibliographique Masereel, Bernard ; Dinguizli, Mustapha ; Bouzin, Caroline ; Moniotte, Nicolas ; Feron, Olivier ; et. al. Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes. In: Journal of Nanoparticle Research : an interdisciplinary forum for nanoscale science and technology, Vol. 13, no. 4, p. 1573-1580 (2011)
Permalien http://hdl.handle.net/2078.1/106182