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Live imaging of mitosomes and hydrogenosomes by HaloTag technology

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Martincová, Eva [Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic] Voleman, Luboš [Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic] Najdrová, Vladimíra [Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic] de Napoli, Maximiliano [Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Chascomús, Provincia de Buenos Aires, Argentina] Eshar, Shiri [Department of Microbiology and Molecular Genetics, The Kuvin Center for the Study of Infectious and Tropical Diseases, IMRIC, The Hebrew University-Hadassah Medical School, Jerusalem, Israel] Gualdron Lopez, Melisa [UCL] Doležal, Pavel [Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic]

Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes. © 2012 Martincová et al.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2012
Language Anglais
Journal information "PLoS One" - Vol. 7, no. 4 (2012)
Peer reviewed yes
issn 1932-6203
e-issn 1932-6203
Publication status Publié
Affiliation UCL - SSS/DDUV - Institut de Duve
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Bibliographic reference Martincová, Eva ; Voleman, Luboš ; Najdrová, Vladimíra ; de Napoli, Maximiliano ; Eshar, Shiri ; et. al. Live imaging of mitosomes and hydrogenosomes by HaloTag technology. In: PLoS One, Vol. 7, no. 4 (2012)
Permanent URL http://hdl.handle.net/2078.1/161483