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Induction of the specific allergic immune response is independent of proteases from the fungus Alternaria alternata

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Denis, Olivier [Program Allergology, WIV-ISP (site Ukkel), Brussels, Belgium] Vincent, Muriel [Program Allergology, WIV-ISP (site Ukkel), Brussels, Belgium] Havaux, Xavier [UCL] De Prins, Sofie [Program Allergology, WIV-ISP (site Ukkel), Brussels, Belgium] Treutens, Greta [Program Allergology, WIV-ISP (site Ukkel), Brussels, Belgium] Huygen, Kris [Scientific Service Immunology, WIV-ISP (site Ukkel), Brussels, Belgium]

We have analyzed the importance of proteases for the induction of allergic responses against the mold Alternaria alternata. Responses induced in vivo with untreated or heat treated (protease inactivated) extracts were compared in BALB/c, C57BL/6, TLR4 KO, and MyD88 KO mice. In BALB/c mice, both extracts induced similar lung inflammation, upregulation of inflammatory mediators, Th2 cytokines, and Alternaria-specific antibodies. However heat inactivation abrogated polyclonal IgE production. Similar results were obtained in C57BL/6 albeit lung expression of some Th2 mediators was decreased in mice stimulated with the heat-treated extract. Treatment of the extract with protease inhibitors did not affect the induction of the allergic response either, except again for the polyclonal IgE response. Th2 responses and lung inflammation were readily induced in TLR4 knockout mice. In contrast, lung inflammation, Th2 responses, cytokine productions, and antibody synthesis were strongly suppressed in MyD88-deficient mice. Early lung IL-33 and IL-1-α expression were also suppressed. In conclusion, albeit some heat labile proteases are required for the stimulation of the polyclonal IgE secretion, fungal proteases, and TLR4 signaling are not required while MyD88 is essential for triggering the systemic immune response and for the development of lung allergic inflammation in response to Alternaria extracts. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2013
Language Anglais
Journal information "European Journal of Immunology" - Vol. 43, no. 4, p. 907-917 (2013)
Peer reviewed yes
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
e-issn 1521-4141
issn 0014-2980
Publication status Publié
Affiliation UCL - SSS/IREC/CARD - Pôle de recherche cardiovasculaire
Links
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Bibliographic reference Denis, Olivier ; Vincent, Muriel ; Havaux, Xavier ; De Prins, Sofie ; Treutens, Greta ; et. al. Induction of the specific allergic immune response is independent of proteases from the fungus Alternaria alternata. In: European Journal of Immunology, Vol. 43, no. 4, p. 907-917 (2013)
Permanent URL http://hdl.handle.net/2078.1/160671