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Cloning and sequencing of a species-specific nucleotide fragment of Borrelia burgdorferi sensu stricto, which is repeated in several plasmids of the species.

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Misonne, M C Schuttler, M Dernelle, J M De Kesel, M Hoet, Philippe [UCL]

Among the etiological agents of Lyme disease, Borrelia burgdorferi sensu stricto strains carry a 16 kb plasmid, which did not hybridize to plasmids of B. garinii and B. afzelii strains. A 1271 bp DNA fragment of the 16 kb plasmid was cloned. It hybridized to several plasmids of this species (16, 27 and 55 kb). Sequencing of the cloned insert revealed a 327 bp ORF coding for a 14 kDa protein of unknown function, which could be expressed in E. coli. This ORF, conserved among B. burgdorferi sensu stricto strains, was carried by the same three plasmids.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1997
Journal information "FEMS microbiology letters" - Vol. 150, no. 1, p. 157-64 (1997)
Peer reviewed yes
issn 0378-1097
Publication status Publié
Affiliation UCL
MESH Subject Amino Acid Sequence ; Base Sequence ; Borrelia burgdorferi ; Borrelia burgdorferi Group - genetics ; Cloning, Molecular ; DNA, Bacterial - analysis - chemistry - genetics ; Escherichia coli - genetics ; Molecular Sequence Data ; Molecular Weight ; Nucleic Acid Hybridization ; Open Reading Frames - genetics ; Plasmids - analysis - chemistry - genetics ; Recombinant Fusion Proteins - chemistry ; Sequence Analysis, DNA ; Species Specificity
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Bibliographic reference Misonne, M C ; Schuttler, M ; Dernelle, J M ; De Kesel, M ; Hoet, Philippe. Cloning and sequencing of a species-specific nucleotide fragment of Borrelia burgdorferi sensu stricto, which is repeated in several plasmids of the species.. In: FEMS microbiology letters, Vol. 150, no. 1, p. 157-64 (1997)
Permanent URL http://hdl.handle.net/2078.1/13187