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A retinoic acid responsive Hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field.

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Diman, Nata Y. S.-G. [UCL] Remacle, Sophie [UCL] Bertrand, Nicolas [AIX-MARSEILLE UNIVERSITY, Marseille, France] Picard, Jacques [UCL] Zaffran, Stéphane [AIX-MARSEILLE UNIVERSITY, Marseille, France] Rezsohazy, René [UCL]

A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm. Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression of the transgene. Therefore, this transgenic line may serve as a cardiosensor line of particular interest for further analysis of outflow tract development.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2011
Language Anglais
Journal information "PLoS One" - Vol. 6, no. 11, p. e27624 (2011)
Peer reviewed yes
Publisher Public Library of Science ((United States) San Francisco, CA)
issn 1932-6203
Publication status Publié
Affiliations UCL - SST/ISV - Institut des sciences de la vie
AIX-MARSEILLE UNIVERSITY, Marseille, France - UMR910
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
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Bibliographic reference Diman, Nata Y. S.-G. ; Remacle, Sophie ; Bertrand, Nicolas ; Picard, Jacques ; Zaffran, Stéphane ; et. al. A retinoic acid responsive Hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field.. In: PLoS One, Vol. 6, no. 11, p. e27624 (2011)
Permanent URL http://hdl.handle.net/2078.1/92677