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In Vitro Dual Culture of Polymyxa betae in Agrobacterium rhizogenes Transformed Sugar Beet Hairy Roots in Liquid Media

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Desoignies, Nicolas [UCL] Legrève, Anne [UCL]

Polymyxa betae is a soil-borne protist and an obligate parasite of sugar beet that transmits the beet necrotic yellow vein virus. Sugar beet hairy roots, transformed by Agrobacterium rhizogenes, were inoculated with surface-sterilized root fragments infected by P. betae. After 10 wk in a liquid medium, typical structures of P. betae were observed in this in vitro system. This first in vitro culture of P. betae in liquid medium will contribute to a better understanding of this protist's biology through providing a way to conserve and produce purified isolates of the protist.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2011
Language Anglais
Journal information "Journal of eukaryotic microbiology" - Vol. 58, no. 5, p. 424-425 (2011)
Peer reviewed yes
issn 1066-5234
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Links
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Bibliographic reference Desoignies, Nicolas ; Legrève, Anne. In Vitro Dual Culture of Polymyxa betae in Agrobacterium rhizogenes Transformed Sugar Beet Hairy Roots in Liquid Media. In: Journal of eukaryotic microbiology, Vol. 58, no. 5, p. 424-425 (2011)
Permanent URL http://hdl.handle.net/2078.1/91754