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Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715

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Höfte, Herman [Plant genetic system, Ghent] de Greve, Henri [Plant genetic system, Ghent] Seurinck, Jef [Plant genetic system, Ghent] Jansens, Stefan [Plant genetic system, Ghent] Mahillon, Jacques [UCL] Vaeck, Mark [Plant genetic system, Ghent]

A plasmid-encoded crystal protein gene (bt2) has been cloned from Bacillus thuringiensis berliner 1715. In Escherichia coli, it directs the synthesis of the 130-kDa protein (Bt2) which is toxic to larvae of Pieris brassicae and Manduca sexta. Comparison of the deduced amino acid sequence of this Bt2 protein with the B. thuringiensis kurstaki HD1 Dipel, B. thuringiensis kurstaki HD73 and B. thuringiensis sotto crystal protein sequences suggests that homologous recombination between the different genes has occurred during evolution. Treatment of the Bt2 protein with trypsin or chymotrypsin yields a 60-kDa protease-resistant and fully toxic polypeptide. The minimal portion of the Bt2 protein required for toxicity has been determined by analysing the polypeptides produced by deletion derivatives of the bt2 gene. It coincides with the 60-kDa protease-resistant Bt2 fragment and it starts between amino acids 29 and 35 at the N-terminus and terminates between positions 599 and 607 at the C-terminus.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1986
Language Anglais
Journal information "European Journal of Biochemistry" - Vol. 161, no. 2, p. 273-280 (1986)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing Ltd. (Berlin ;)
issn 0014-2956
e-issn 1432-1033
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Links
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Bibliographic reference Höfte, Herman ; de Greve, Henri ; Seurinck, Jef ; Jansens, Stefan ; Mahillon, Jacques ; et. al. Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715. In: European Journal of Biochemistry, Vol. 161, no. 2, p. 273-280 (1986)
Permanent URL http://hdl.handle.net/2078.1/90576