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Effect in vitro and in vivo of a rat anti-CD2 monoclonal antibody (LO-CD2b) on pig-to-baboon xenogeneic cellular (T and natural killer cells) immune response.

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Dehoux, Jean-Paul [UCL] de la Parra, Bernardo [UCL] Latinne, Dominique [UCL] Bazin, Hervé [UCL] Gianello, Pierre [UCL]

Although hyperacute rejection of discordant xenogeneic grafts can be prevented, baboon or human anti-pig cellular response may lead to acute xenograft rejection. Among the immune cellular actors participating in such a xenograft rejection are both T and natural killer (NK) cells. In the pre-clinical model of pig-to-baboon discordant xenograft, there is however, a lack of specific immunological therapeutic agent, in particular antibaboon T-cell monoclonal antibodies do not exist. We therefore developed a rat anti-CD2 monoclonal antibody (LO-CD2b) that recognizes both baboon and human CD2 + cells. In this study, we show that in vitro LO-CD2b inhibits a pig-to-baboon mixed lymphocyte reaction, the direct cytotoxicity of baboon peripheral blood lymphocytes to pig aortic endothelial cells, as well as the baboon NK activity against K562 cell line. In vivo, LO-CD2b produces a strong depletion of all peripheral CD2+ cells including NK CD2+ cells. In summary, LO-CD2b represents an important immunological tool that can be used in the preclinical model of discordant pig-to-baboon vascularized xenograft.

  1. Yamada K, J Immunol, 155, 5249 (1995)
  2. Platt JL, Xenotransplantation, 5, 169 (1998)
  3. Satake M, Xenotransplantation, 3 (1996)
  4. Platt JL, Transplantation, 52, 214 (1991)
  5. Cunningham Anne, Future developments in transplantation, 10.1016/0966-3274(94)90054-x
  6. Itescu S., Kwiatkowski P., Artrip J.H., Wang S.F., Ankersmit J., Minanov O.P., Michler R.E., Role of Natural Killer Cells, Macrophages, and Accessory Molecule Interactions in the Rejection of Pig-to-Primate Xenografts Beyond the Hyperacute Period, 10.1016/s0198-8859(98)00026-3
  7. Cooke SP, Pearson JD, Savage COS, Human IgG xenoreactive antibodies mediate damage to porcine endothelial cells in vitro by both humoral and cellular mechanisms, 10.1016/s0966-3274(97)80024-x
  8. Sasaki Hitomi, Xu Xiao-Chun, Smith Douglas M., Howard Todd, Mohanakumar T., HLA-G EXPRESSION PROTECTS PORCINE ENDOTHELIAL CELLS AGAINST NATURAL KILLER CELL-MEDIATED XENOGENEIC CYTOTOXICITY1,2 : , 10.1097/00007890-199901150-00005
  9. Shishido Seiichiro, Naziruddin Bashoo, Howard Todd, Mohanakumar T., RECOGNITION OF PORCINE MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I ANTIGENS BY HUMAN CD8+ CYTOLYTIC T CELL CLONES1 : , 10.1097/00007890-199707270-00028
  10. Goodman David J., von Albertini Michaela, Willson Amy, Millan Maria T., Bach Fritz H., DIRECT ACTIVATION OF PORCINE ENDOTHELIAL CELLS BY HUMAN NATURAL KILLER CELLS1 : , 10.1097/00007890-199603150-00016
  11. Bazin H, Production of Rat Monoclonal Antibodies with LOU Rat Non Secreting IR983F Myeloma Cell Line. (1992)
  12. Xia H, Rat Hybridomas and Rat Monoclonal Antibodies. (1990)
  13. Bazin H, Rat Hybridomas and Rat Monoclonal Antibodies. (1990)
  14. Seebach JD, Xenotransplantation, 4, 201 (1997)
  15. Rappocciolo G, Vet Pathol, 29, 53 (1992)
  16. Lanier Lewis L., NK CELL RECEPTORS, 10.1146/annurev.immunol.16.1.359
  17. Ravetch J., FC-Receptors, 10.1146/annurev.immunol.9.1.457
  18. Dehoux Jean-Paul, Talpe St??phanie, Dewolf Natacha, Otsuka Masayuki, Oike Fumitaka, Jamar Fran??ois, de la Parra Bernardo, Latinne Dominique, Bazin Herv??, Gianello Pierre, EFFECTS ON HUMAN AND NONHUMAN PRIMATE IMMUNE RESPONSE OF A NEW RAT ANTI-CD2 MONOCLONAL ANTIBODY1 : , 10.1097/00007890-200006270-00024
  19. Seebach JD, Xenotransplantation., 3, 188 (1996)
  20. Manilay Jennifer O, Sykes Megan, Natural killer cells and their role in graft rejection, 10.1016/s0952-7915(98)80219-7
  21. Pleass Henry C.C., Forsythe John L.R., Proud George, Taylor R.M.R., Kirby John A., Xenotransplantation: an examination of the adhesive interactions between human lymphocytes and porcine renal epithelial cells, 10.1016/0966-3274(94)90064-7
Bibliographic reference Dehoux, Jean-Paul ; de la Parra, Bernardo ; Latinne, Dominique ; Bazin, Hervé ; Gianello, Pierre. Effect in vitro and in vivo of a rat anti-CD2 monoclonal antibody (LO-CD2b) on pig-to-baboon xenogeneic cellular (T and natural killer cells) immune response.. In: Xenotransplantation, Vol. 8, no. 3, p. 193-201 (2001)
Permanent URL http://hdl.handle.net/2078.1/8899