User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype.

Primary tabs

Deneys, Véronique [UCL] Thiry, V Hougardy, N. Mazzon, A M Leveugle, P De Bruyère, Marc [UCL]

BACKGROUND AND OBJECTIVES: Freezing is a practical approach for cell preservation for retrospective studies. The aim of this work was to check the cryopreservation impact on B cell chronic lymphocytic leukaemia phenotype. MATERIAL AND METHODS: Blood samples from 15 CLL patients were analyzed freshly and after freezing at -196 degrees C, without separation, and thawing. Results were compared by Student's paired t-test. RESULTS: The phenotype of fresh CLL cells was as follows: CD19+, CD5+, faint CD20, CD23+/-, weak CD22 and sIg, CD37+, HLA-DR+, FMC7-. After cryopreservation, the percentage of CD5 and CD23 positive cells decreased, whereas HLA-DR positive cells increased moderately. The CLL Matutes's score was modified in 6 cases out of 15 (40%). CONCLUSION: Cryopreservation modifies B cell chronic lymphocytic leukaemia phenotype, by decreasing CD5 and CD23 expression.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1999
Journal information "Journal of immunological methods" - Vol. 228, no. 1-2, p. 13-21 (1999)
Peer reviewed yes
issn 0022-1759
Publication status Publié
Affiliation UCL - MD/MINT - Département de médecine interne
MESH Subject Adult ; Antigens, CD - blood ; Antigens, CD5 - blood ; B-Lymphocytes - immunology ; Blood Preservation ; Cryopreservation ; Evaluation Studies as Topic ; Flow Cytometry ; HLA-DR Antigens - blood ; Humans ; Immunoglobulin kappa-Chains - blood ; Immunoglobulin lambda-Chains - blood ; Immunophenotyping ; Leukemia, B-Cell - blood - immunology ; Receptors, IgE - blood
Links
Bibliographic reference Deneys, Véronique ; Thiry, V ; Hougardy, N. ; Mazzon, A M ; Leveugle, P ; et. al. Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype.. In: Journal of immunological methods, Vol. 228, no. 1-2, p. 13-21 (1999)
Permanent URL http://hdl.handle.net/2078.1/8403