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Five challenges to bringing single-molecule force spectroscopy into living cells.

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Dufrêne, Yves [UCL] Evans, Evan [Boston University, Boston Massachussetts, USA] Engel, Andreas [Case Western Reserve University, Cleveland, Ohio, USA] Helenius, Jonne [Eidgenössische Technische Hochschule Zürich, Basel, Switzerland] Gaub, Herman E. [Ludwig Maximillians University, Munich, Germany] Müller, Daniel J. [Eidgenössische Technische Hochschule Zürich, Basel, Switzerland]

In recent years, single-molecule force spectroscopy techniques have been used to study how inter- and intramolecular interactions control the assembly and functional state of biomolecular machinery in vitro. Here we discuss the problems and challenges that need to be addressed to bring these technologies into living cells and to learn how cellular machinery is controlled in vivo.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2011
Language Anglais
Journal information "Nature Methods : techniques for life scientists and chemists" - Vol. 8, no. 2, p. 123-127 (2011)
Peer reviewed yes
Publisher Nature Publishing Group ((United Kingdom) London)
issn 1548-7091
e-issn 1548-7105
Publication status Publié
Affiliations UCL - SST/IMCN/BSMA - Bio and soft matter
Case Western Reserve University, Cleveland, Ohio, USA - Department of Pharmacology
Boston University, Boston Massachussetts, USA - Medical Engineering and Physics
Eidgenössische Technische Hochschule Zürich, Basel, Switzerland - Department of Biosystems Science and Engineering
Ludwig Maximillians University, Munich, Germany - Applied Physics
Links
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Bibliographic reference Dufrêne, Yves ; Evans, Evan ; Engel, Andreas ; Helenius, Jonne ; Gaub, Herman E. ; et. al. Five challenges to bringing single-molecule force spectroscopy into living cells.. In: Nature Methods : techniques for life scientists and chemists, Vol. 8, no. 2, p. 123-127 (2011)
Permanent URL http://hdl.handle.net/2078.1/75163