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The Sequence of An 8.8-kb Segment On the Left Arm of Chromosome-ii From Saccharomyces-cerevisiae Reveals 4 New Open Reading Frames Including Homologs of Animal Dna-polymerase Alpha-primases and Bacterial Gtp Cyclohydrolase-ii

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Skala, J. Vandyck, L. Purnelle, Bénédicte [UCL] Goffeau, André [UCL]

The DNA sequence of two contiguous 7648 bp and 1194 bp BamHI fragments from the cosmid alpha 1201 located about 60 kb from the centromere on the left arm of chromosome II from Saccharomyces cerevisiae has been determined. Sequence analysis reveals four new open reading frames longer than 300 bp: YBL0415 (309 bp), YBL0416(4539 bp), YBL0417 (1035 bp) and YBL0414 (2115 bp), which extends into the neighbouring 5.2 kb BamHI fragment. The YBL0414 shows homologies to the mouse 68 kDa and Drosophila melanogaster 76 kDa subunits of the DNA polymerase alpha-primase complex. The YBL0417 is homologous to bacterial GTP cyclohydrolase II (EC 3.5.4.25). The sequence has been deposited in the EMBC data library under Accession Number X74738.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 1994
Language Anglais
Journal information "Yeast : a forum for yeast researchers" - Vol. 10, p. S13-S24 (1994)
Peer reviewed yes
Publisher John Wiley & Sons Ltd (W Sussex)
issn 0749-503X
e-issn 1097-0061
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
Keywords YEAST ; SACCHAROMYCES CEREVISIAE ; CHROMOSOME II ; POLYMERASE ALPHA-PRIMASE ; CYCLOHYDROLASE II ; RIBA ; DNA SEQUENCING
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Bibliographic reference Skala, J. ; Vandyck, L. ; Purnelle, Bénédicte ; Goffeau, André. The Sequence of An 8.8-kb Segment On the Left Arm of Chromosome-ii From Saccharomyces-cerevisiae Reveals 4 New Open Reading Frames Including Homologs of Animal Dna-polymerase Alpha-primases and Bacterial Gtp Cyclohydrolase-ii. In: Yeast : a forum for yeast researchers, Vol. 10, p. S13-S24 (1994)
Permanent URL http://hdl.handle.net/2078.1/48947