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Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936

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Evans, P. A. S.

Polymerase chain reaction (PCR) assessment of clonal immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements is an important diagnostic tool in mature B-cell neoplasms. However, lack of standardized PCR protocols resulting in a high level of false negativity has hampered comparability of data in previous clonality studies. In order to address these problems, 22 European laboratories investigated the Ig/TCR rearrangement patterns as well as t(14; 18) and t( 11; 14) translocations of 369 B-cell malignancies belonging to five WHO-defined entities using the standardized BIOMED-2 multiplex PCR tubes accompanied by international pathology panel review. B-cell clonality was detected by combined use of the IGH and IGK multiplex PCR assays in all 260 definitive cases of B-cell chronic lymphocytic leukemia (n = 56), mantle cell lymphoma ( n 54), marginal zone lymphoma ( n 41) and follicular lymphoma ( n 109). Two of 109 cases of diffuse large B-cell lymphoma showed no detectable clonal marker. The use of these techniques to assign cell lineage should be treated with caution as additional clonal TCR gene rearrangements were frequently detected in all disease categories. Our study indicates that the BIOMED-2 multiplex PCR assays provide a powerful strategy for clonality assessment in B-cell malignancies resulting in high Ig clonality detection rates particularly when IGH and IGK strategies are combined.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2007
Language Anglais
Journal information "Leukemia" - Vol. 21, no. 2, p. 207-214 (2007)
Peer reviewed yes
Publisher Nature Publishing Group (London)
issn 0887-6924
e-issn 1476-5551
Publication status Publié
Affiliation UCL
Keywords clonality ; PCR ; B-cell malignancies ; Ig rearrangements ; TCR rearrangements ; BIOMED-2
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Bibliographic reference Evans, P. A. S. ; et. al. Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936. In: Leukemia, Vol. 21, no. 2, p. 207-214 (2007)
Permanent URL http://hdl.handle.net/2078.1/37800