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Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from Calycotome villosa in rats

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Cherkaoui-Tangi, Khadija [UCL] Lachkar, M. Wibo, Maurice [UCL] Morel, Nicole [UCL] Gilani, A. H. Lyoussi, B.

The present study was undertaken in normotensive anaesthetized male rats that received a continuous perfusion of a chrysin glucoside isolated from the flowers and leaves of Calycotome villosa subsp intermedia at a dose of 2.5 mg/kg, or furosemide (control diuretic) at a dose of 0.5 mg/kg. Compared with the control rats receiving NaCl (0.9%), the urine flow, glomerular filtration and electrolyte excretion (Na+, K+) increased significantly in rats treated with chrysin glucoside (p < 0.001). A similar effect was observed in the rats perfused with furosemide. Intravenous injections of bolus doses (1-3 mg/kg) of the chrysin glucoside to anaesthetized rats elicited an immediate and dose-dependent decrease in mean arterial blood pressure (MABP). Pretreatment of the rats with the nitric oxide synthase inhibitor, L-NOArg (10 mg/kg), reduced partially, but significantly (p < 0.01), the maximal decrease in MABP elicited by chrysin glucoside. In the rat isolated aorta preparation, chrysin glucoside (10-100 mu m) inhibited in a concentration-dependent manner the noradrenaline (1 mu m) induced contractions (IC50 = 52 mu m). This relaxant activity of chrysin glucoside was significantly reduced by incubation of the endothelim-intact rings with L-NOArg (100 mu m), (80 +/- 4.7% vs 48 +/- 5.06% in the absence of L-NOArg). In conclusion, these results demonstrate a diuretic and hypotensive action of a chrysin glucoside from Calycotome villosa in anaesthetized rats and indicating an action on renal function, and an active vascular relaxation mediated partially through nitric oxide release. Copyright (C) 2007 John Wiley & Sons, Ltd.

Document type Article de périodique (Journal article) – Journal Article
Access type Accès restreint
Publication date 2008
Language Anglais
Journal information "Phytotherapy Research : an international journal devoted to medical and scientific research on plants and plant products" - Vol. 22, no. 3, p. 356-61 (2008)
Peer reviewed yes
Publisher John Wiley & Sons Ltd (Chichester)
issn 0951-418X
e-issn 1099-1573
Publication status Publié
Affiliation UCL - MD/FSIO - Département de physiologie et pharmacologie
MESH Subject Water-Electrolyte Balance - drug effects ; Vasodilator Agents - chemistry - pharmacology ; Time Factors ; Rats, Wistar ; Rats ; Plant Extracts - chemistry - pharmacology ; Phytotherapy ; Male ; Kidney - drug effects ; Glucosides - chemistry ; Furosemide - pharmacology ; Flavonoids - chemistry - pharmacology ; Fabaceae - chemistry ; Diuretics - chemistry - pharmacology ; Diuresis - drug effects ; Blood Pressure - drug effects ; Aorta, Thoracic - drug effects ; Antihypertensive Agents - chemistry - pharmacology ; Animals
Keywords chrysin glucoside ; Calycotome villosa ; diuretic ; hypotensive ; vasorelaxant ; endothelial dependent ; rats
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Bibliographic reference Cherkaoui-Tangi, Khadija ; Lachkar, M. ; Wibo, Maurice ; Morel, Nicole ; Gilani, A. H. ; et. al. Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from Calycotome villosa in rats. In: Phytotherapy Research : an international journal devoted to medical and scientific research on plants and plant products, Vol. 22, no. 3, p. 356-61 (2008)
Permanent URL http://hdl.handle.net/2078.1/36710