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Lignocellulose-degrading enzyme production by white-rot Basidiomycetes isolated from the forests of Georgia

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Elisashvili, Vladimir Kachlishvili, Eva Tsiklauri, Nino Metreveli, Eka Khardziani, Tamar Agathos, Spiros N. [UCL]

The production of lignocellulolytic enzymes by eleven basidiomycetes species isolated from two ecosystems of Georgia was investigated for the first time under submerged (SF) and solid-state fermentation (SSF) of lignocellulosic by-products. Notable intergeneric and intrageneric differences were revealed with regard to the extent of hydrolase and oxidase activity. Several fungi produced laccase along with hydrolases in parallel with growth during the trophophase, showing that the synthesis of this enzyme is not connected with secondary metabolism. The lignocellulosic substrate type had the greatest impact on enzyme secretion. Some of the substrates significantly stimulated lignocellulolytic enzyme synthesis without supplementation of the culture medium with specific inducers. Exceptionally high carboxymethyl cellulase (CMCase, 122 U ml(-1)) and xylanase (195 U ml(-1)) activities were revealed in SF of mandarin peelings by Pseudotremella gibbosa IBB 22 and of residue after ethanol production (REP) by Fomes fomentarius IBB 38, respectively. The SSF of REP by T. pubescens IBB 11 ensured the highest level of laccase activity (24,690 U l(-1)), whereas the SSF of wheat bran and SF of mandarin peels provided the highest manganese peroxidase activity (570-620 U l(-1)) of Trichaptum biforme IBB 117. Moreover, the variation of lignocellulosic growth substrate provides an opportunity to obtain enzyme preparations containing different ratios of individual enzymes.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2009
Language Anglais
Journal information "World Journal of Microbiology and Biotechnology" - Vol. 25, no. 2, p. 331-339 (2009)
Peer reviewed yes
Publisher Springer (New York)
issn 0959-3993
e-issn 1573-0972
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
Keywords White-rot Basidiomycetes ; Cellulase ; Laccase ; Lignocellulose fermentation ; Manganese peroxidase ; Xylanase
Links
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Bibliographic reference Elisashvili, Vladimir ; Kachlishvili, Eva ; Tsiklauri, Nino ; Metreveli, Eka ; Khardziani, Tamar ; et. al. Lignocellulose-degrading enzyme production by white-rot Basidiomycetes isolated from the forests of Georgia. In: World Journal of Microbiology and Biotechnology, Vol. 25, no. 2, p. 331-339 (2009)
Permanent URL http://hdl.handle.net/2078.1/35774