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Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis

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Van Keymeulen, Alexandra Mascre, Guilhem Youseff, Khalil Kass Harel, Itamar Michaux, Cindy De Geest, Natalie [UCL] Achouri, Younes [UCL] Hassan, Bassem A. [UCL] Blanpain, Cedric

Merkel cells (MCs) are located in the touch-sensitive area of the epidermis and mediate mechano-transduction in the skin. Whether MCs originate from embryonic epidermal or neural crest progenitors has been a matter of intense controversy since their discovery > 130 yr ago. In addition, how MCs are maintained during adulthood is currently unknown. In this study, using lineage-tracing experiments, we show that MCs arise through the differentiation of epidermal progenitors during embryonic development. In adults, MCs undergo slow turnover and are replaced by cells originating from epidermal stem cells, not through the proliferation of differentiated MCs. Conditional deletion of the Atoh1/Math1 transcription factor in epidermal progenitors results in the absence of MCs in all body locations, including the whisker region. Our study demonstrates that MCs arise from the epidermis by an Atoh1-dependent mechanism and opens new avenues for study of MC functions in sensory perception, neuroendocrine signaling, and MC carcinoma.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 2009
Language Anglais
Journal information "The Journal of Cell Biology" - Vol. 187, no. 1, p. 91-100 (2009)
Peer reviewed yes
Publisher Rockefeller Univ Press (New York)
issn 0021-9525
e-issn 1540-8140
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Vibrissae - cytology - embryology - metabolism ; Time Factors ; Stem Cells - cytology ; Skin - cytology - embryology - metabolism - ultrastructure ; Neurofilament Proteins - genetics - metabolism ; Neural Crest - cytology - embryology ; Mice, Transgenic ; Mice, Knockout ; Mice ; Merkel Cells - cytology - metabolism - physiology ; Integrases - genetics - metabolism ; Immunohistochemistry ; Homeostasis ; Fluorescent Antibody Technique, Direct ; Epidermis - cytology - metabolism - ultrastructure ; Cell Lineage ; Cell Differentiation ; Cadherins - metabolism ; Biological Markers - metabolism ; Basic Helix-Loop-Helix Transcription Factors - genetics - metabolism ; Animals, Newborn ; Animals ; Aging
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Bibliographic reference Van Keymeulen, Alexandra ; Mascre, Guilhem ; Youseff, Khalil Kass ; Harel, Itamar ; Michaux, Cindy ; et. al. Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis. In: The Journal of Cell Biology, Vol. 187, no. 1, p. 91-100 (2009)
Permanent URL http://hdl.handle.net/2078.1/35272