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A mutated intron sequence codes for an antigenic peptide recognized by cytolytic T lymphocytes on a human melanoma.

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Coulie, Pierre G. [UCL] Lehmann, F. Lethé, B Lurquin, C. Andrawiss, M Boon, Thierry [UCL] Herman, Jacques [UCL]

We have identified an antigen recognized on a human melanoma by autologous cytolytic T lymphocytes. It is encoded by a gene that is expressed in many normal tissues. Remarkably, the sequence coding for the antigenic peptide is located across an exon-intron junction. A point mutation is present in the intron that generates an amino acid change that is essential for the recognition of the peptide by the anti-tumor cytotoxic T lymphocytes. This observation suggests that the T-cell-mediated surveillance of the integrity of the genome may extend to some intronic regions.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 1995
Journal information "Proceedings of the National Academy of Sciences of the United States of America" - Vol. 92, no. 17, p. 7976-80 (1995)
Peer reviewed yes
issn 0027-8424
Publication status Publié
Affiliation UCL
MESH Subject Alleles ; Amino Acid Sequence ; Antigens, Neoplasm - genetics - immunology ; Base Sequence ; Binding, Competitive ; Cell Line ; Cloning, Molecular ; Cytotoxicity, Immunologic ; Exons ; Humans ; Introns ; Kinetics ; Melanoma - immunology ; Molecular Sequence Data ; Mutagenesis ; Neoplasm Proteins - biosynthesis - genetics - immunology ; Point Mutation ; Recombinant Proteins - immunology ; T-Lymphocytes, Cytotoxic - immunology ; Transfection ; Tumor Cells, Cultured
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Bibliographic reference Coulie, Pierre G. ; Lehmann, F. ; Lethé, B ; Lurquin, C. ; Andrawiss, M ; et. al. A mutated intron sequence codes for an antigenic peptide recognized by cytolytic T lymphocytes on a human melanoma.. In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, no. 17, p. 7976-80 (1995)
Permanent URL http://hdl.handle.net/2078.1/29600