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A comparative analysis of alterations in protein expression after activation or human immunodeficiency virus, type 1 infection of human CD4+ T cells.

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Van de Keere, Fabienne [UCL] Willard-Gallo, Karen E. [UCL]

We have previously described an in vitro model for studying human immunodeficiency virus, type 1 (HIV-1) infection in CD4+ T cells [1]. This model employs the WE17/10 cell line, which loses expression of its T cell receptor/CD3 (TCR/CD3) after several months of productive infection. We have used this model to analyze the synthesis and posttranslational modification of viral and cellular proteins after HIV-1 infection and to determine the relationship of these changes to TCR/CD3 expression. Mainly we observe positive changes in protein expression after infection. A phosphoprotein, referred to as WH:1, appears in infected cells that still express their TCR/CD3 complex, and its persistence is linked to the presence of the complex. We examined whether loss of the TCR/CD3 complex could be associated with alterations in the T cell activation pathway as a result of infection. We used T cell activators and inhibitors to determine whether there were common elements between the two events. Quantitative enhancement in one spot, Cs:1, occurred after both Cyclosporin A treatment of uninfected cells and HIV-1 infection of untreated cells. Taken altogether, these data suggest that a correlation exists between negative regulation of late events in the T cell activation pathway and down regulation of the TCR/CD3 complex after HIV-1 infection.

Document type Article de périodique (Journal article) – Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Publication date 1991
Journal information "Electrophoresis" - Vol. 12, no. 7-8, p. 544-53 (1991)
Peer reviewed yes
issn 0173-0835
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Alkaloids - pharmacology ; Antigens, CD - analysis ; Antigens, CD4 - analysis - immunology ; Autoradiography - methods ; Cell Line ; Cyclosporine - pharmacology ; Electrophoresis, Gel, Two-Dimensional - methods ; HIV-1 - immunology ; HLA-D Antigens - analysis ; Histocompatibility Antigens Class I - analysis ; Humans ; Lymphocyte Activation ; Protein Kinase C - antagonists & inhibitors ; Staurosporine ; Sulfur Radioisotopes ; T-Lymphocyte Subsets - chemistry - drug effects - immunology ; Tetradecanoylphorbol Acetate - pharmacology
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Bibliographic reference Van de Keere, Fabienne ; Willard-Gallo, Karen E.. A comparative analysis of alterations in protein expression after activation or human immunodeficiency virus, type 1 infection of human CD4+ T cells.. In: Electrophoresis, Vol. 12, no. 7-8, p. 544-53 (1991)
Permanent URL http://hdl.handle.net/2078.1/29390