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The tumor protein MAGE-1 is located in the cytosol of human melanoma cells

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Amar-Costesec, Alain Godelaine, Danièle [UCL] Stockert, Elisabeth Van Der Bruggen, Pierre [UCL] Beaufay, Henri [UCL] Chen, Yao-Tseng

The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribution between nuclear, mitochondrial, microsomal and cytosolic fractions was established by SDS-PAGE and immunoblotting with a rabbit antiserum and compared to that of markers for the main cell compartments. The immunoreactive material was recovered in the cytosolic fraction as a polypeptide migrating at 42 kDa, which has been further identified as the MAGE-1 protein because it comigrates with the product of cell-free transcription-translation of the MAGE-1 cDNA. Location to the cytosol was confirmed by immunofluorescence microscopy.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1994
Journal information "Biochemical and Biophysical Research Communications" - Vol. 204, no. 2, p. 710-715 (1994)
Peer reviewed yes
Publisher Elsevier Inc. (Philadelphia)
issn 0006-291X
e-issn 1090-2104
Publication status Publié
Affiliation UCL - (SLuc) Centre du cancer
MESH Subject Antigens, Neoplasm - genetics - metabolism ; Cytosol - metabolism ; DNA, Complementary ; Humans ; Melanoma - metabolism ; Microscopy, Fluorescence ; Neoplasm Proteins ; Protein Biosynthesis ; Subcellular Fractions - metabolism ; Transcription, Genetic ; Tumor Cells, Cultured
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Bibliographic reference Amar-Costesec, Alain ; Godelaine, Danièle ; Stockert, Elisabeth ; Van Der Bruggen, Pierre ; Beaufay, Henri ; et. al. The tumor protein MAGE-1 is located in the cytosol of human melanoma cells. In: Biochemical and Biophysical Research Communications, Vol. 204, no. 2, p. 710-715 (1994)
Permanent URL http://hdl.handle.net/2078.1/29372