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Toward prevention of cowdriosis.

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Pedregal, Andreia de Sousa, Daniel Ribeiro Nguyen, Hoang Nam das Neves, Eane Agapito Lowagie, Serge Marique, Thierry [UCL] Wérenne, John

Mass production of Ehrlichia ruminantium variants from different regions of sub-Saharan Africa is one of the difficulties that must be overcome in producing a heartwater vaccine. Vaccine productivity can be limited by endogenous induction of interferon (IFN), which inhibits the propagation of Ehrlichia ruminantium (ER) in cell culture. Different kinds of endothelial cells, in which ER multiply efficiently, could be grown in a scalable way in VueLife Teflon bags on Cytodex 3 microcarriers where bead-to-bead transfer of cells occurs. The digital holographic microscope designed at the Université Libre de Bruxelles allows detection of the most appropriate time to harvest intracellular microorganisms for vaccine production.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 2008
Journal information "Annals of the New York Academy of Sciences" - Vol. 1149, p. 286-91 (2008)
Peer reviewed yes
issn 0077-8923
e-issn 1749-6632
Affiliation UCL - MD/IEPR - Institut d'éducation physique et de réadaptation
MESH Subject Animals ; Bacterial Vaccines - therapeutic use ; Cattle ; Ehrlichiosis - prevention & control ; Humans ; Hybrid Cells ; Interferons - metabolism
Links
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Bibliographic reference Pedregal, Andreia ; de Sousa, Daniel Ribeiro ; Nguyen, Hoang Nam ; das Neves, Eane Agapito ; Lowagie, Serge ; et. al. Toward prevention of cowdriosis.. In: Annals of the New York Academy of Sciences, Vol. 1149, p. 286-91 (2008)
Permanent URL http://hdl.handle.net/2078.1/28106