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Maize black Mexican sweet suspension cultured cells are a convenient tool for studying aquaporin activity and regulation

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Cavez, Damien [UCL] Hachez, Charles [UCL] Chaumont, François [UCL]

Aquaporins (AQPs) are channel proteins that facilitate and regulate the permeation of water across biological membranes. Black mMexican sweet suspension cultured cells are a convenient model for studying the regulation of maize AQP expression and activity. Among other advantages, a single cell system allows the contribution of plasma membrane AQPs (PIPs, plasma membrane intrinsic proteins) to the membrane water permeability coefficient (P(f)) to be determined using biophysical measurement methods, such as the cell pressure probe or protoplast swelling assay. We generated a transgenic cell culture line expressing a tagged version of ZmPIP2;6 and used this material to demonstrate that the ZmPIP2;6 and ZmPIP2;1 isoforms physically interact. This kind of interaction could be an additional mechanism for regulating membrane water permeability by acting on the activity and/or trafficking of PIP hetero-oligomers.

Document type Article de périodique (Journal article) – Article de recherche – Comment, Journal Article
Publication date 2009
Language Anglais
Journal information "Plant Signalling & Behavior" - Vol. 4, no. 9, p. 890-2 (2009)
Peer reviewed yes
Publisher Landes Bioscience ((United States) Austin)
issn 1559-2316
e-issn 1559-2324
Publication status Publié
Affiliations UCL - SC/BIOL - Département de biologie
UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
Links
  1. Chaumont F., Aquaporins Constitute a Large and Highly Divergent Protein Family in Maize, 10.1104/pp.125.3.1206
  2. Chaumont François, Barrieu François, Jung Rudolf, Chrispeels Maarten J., Plasma Membrane Intrinsic Proteins from Maize Cluster in Two Sequence Subgroups with Differential Aquaporin Activity, 10.1104/pp.122.4.1025
  3. Zelazny E., Borst J. W., Muylaert M., Batoko H., Hemminga M. A., Chaumont F., FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization, 10.1073/pnas.0701180104
  4. Fetter K., Interactions between Plasma Membrane Aquaporins Modulate Their Water Channel Activity, 10.1105/tpc.017194
  5. Chaumont François, Moshelion Menachem, Daniels Mark J., Regulation of plant aquaporin activity, 10.1042/bc20040133
  6. LUU DOAN-TRUNG, MAUREL CHRISTOPHE, Aquaporins in a challenging environment: molecular gears for adjusting plant water status, 10.1111/j.1365-3040.2004.01295.x
  7. Maurel Christophe, Verdoucq Lionel, Luu Doan-Trung, Santoni Véronique, Plant Aquaporins: Membrane Channels with Multiple Integrated Functions, 10.1146/annurev.arplant.59.032607.092734
  8. KOBAE Yoshihiro, MIZUTANI Masahiro, SEGAMI Shoji, MAESHIMA Masayoshi, Immunochemical Analysis of Aquaporin Isoforms inArabidopsisSuspension-Cultured Cells, 10.1271/bbb.70.980
  9. Moshelion M, Plant Cell Environ (2009)
  10. Gerbeau Patricia, Amodeo Gabriela, Henzler Tobias, Santoni Veronique, Ripoche Pierre, Maurel Christophe, The water permeability of Arabidopsis plasma membrane is regulated by divalent cations and pH, 10.1046/j.1365-313x.2002.01268.x
  11. Hachez Charles, Moshelion Menachem, Zelazny Enric, Cavez Damien, Chaumont François, Localization and Quantification of Plasma Membrane Aquaporin Expression in Maize Primary Root: A Clue to Understanding their Role as Cellular Plumbers, 10.1007/s11103-006-9022-1
  12. Prak Sodana, Hem Sonia, Boudet Julie, Viennois Gaëlle, Sommerer Nicolas, Rossignol Michel, Maurel Christophe, Santoni Véronique, Multiple Phosphorylations in the C-terminal Tail of Plant Plasma Membrane Aquaporins : Role in Subcellular Trafficking ofAtPIP2;1 in Response to Salt Stress, 10.1074/mcp.m700566-mcp200
  13. Van Wilder Valérie, Miecielica Urszula, Degand Hervé, Derua Rita, Waelkens Etienne, Chaumont François, Maize Plasma Membrane Aquaporins Belonging to the PIP1 and PIP2 Subgroups are in vivo Phosphorylated, 10.1093/pcp/pcn112
  14. Christensen Alan H., Quail Peter H., Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants, 10.1007/bf01969712
Bibliographic reference Cavez, Damien ; Hachez, Charles ; Chaumont, François. Maize black Mexican sweet suspension cultured cells are a convenient tool for studying aquaporin activity and regulation. In: Plant Signalling & Behavior, Vol. 4, no. 9, p. 890-2 (2009)
Permanent URL http://hdl.handle.net/2078.1/27166