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The crystal structure of glucose-6-phosphate isomerase from Leishmania mexicana reveals novel active site features.

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Cordeiro, Artur T Michels, Paulus [UCL] Delboni, Luiz F Thiemann, Otávio H

Glucose-6-phosphate isomerase catalyzes the reversible aldose-ketose isomerization of D-glucose-6-phosphate to D-fructose-6-phosphate in glycolysis and gluconeogenesis, and in the recycling of hexose-6-phosphate in the pentose phosphate pathway. The unicellular protozoans, Trypanosoma brucei, T. cruzi and Leishmania spp., of the order Kinetoplastida are important human parasites responsible for African sleeping sickness, Chagas' disease and leishmaniases, respectively. In these parasites, glycolysis is an important (and in some cases the only) metabolic pathway for ATP supply. The first seven of the 10 enzymes that participate in glycolysis, as well as an important fraction of the enzymes of the pentose phosphate pathway, are compartmentalized in peroxisome-like organelles called glycosomes. The dependence of the parasites on glycolysis, the importance of the pentose phosphate pathway in defense against oxidative stress, and the unique compartmentalization of these pathways, point to the enzymes contained in the glycosome as potential targets for drug design. The present report describes the first crystallographic structure of a parasite (Leishmania mexicana) glucose-6-phosphate isomerase. A comparison of the atomic structure of L. mexicana, human and other mammalian PGIs, which highlights unique features of the parasite's enzyme, is presented.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Access type Accès restreint
Publication date 2004
Journal information "European journal of biochemistry / FEBS" - Vol. 271, no. 13, p. 2765-72 (2004)
Peer reviewed yes
issn 0014-2956
Publication status Publié
Affiliation UCL
MESH Subject Amino Acid Sequence ; Animals ; Binding Sites ; Crystallography, X-Ray ; Glucose-6-Phosphate Isomerase - chemistry - metabolism ; Humans ; Leishmania mexicana - enzymology ; Models, Molecular ; Molecular Sequence Data ; Protein Conformation ; Sequence Homology, Amino Acid
Links
  1. Olliaro, Parasitol. Today, 9, 323 (1993)
  2. Verlinde, Drug Resist. Updates, 4, 50 (2001)
  3. Michels, Parasitol. Today, 16, 482 (2000)
  4. Heise, Mol. Biochem. Parasitol., 99, 21 (1999)
  5. Duffieux, J. Biol. Chem., 275, 27559 (2000)
  6. Barrett, Parasitol. Today, 13, 11 (1997)
  7. Barrett, Curr. Top. Med. Chem., 2, 471 (2002)
  8. Nyame, Mol. Biochem. Parasitol., 67, 269 (1994)
  9. Marchand, Eur. J. Biochem., 184, 455 (1989)
  10. Cazzulo, FASEB J., 6, 3153 (1992)
  11. Cronin, FEBS Lett., 244, 26 (1989)
  12. Maugeri, Mol. Biochem. Parasitol., 130, 117 (2003)
  13. Redmond, Mol. Biochem. Parasitol., 128, 115 (2003)
  14. Cordeiro, Acta Crystallogr. D. Biol. Crystallogr., 60, 915 (2004)
  15. Otwinowski, Methods Enzymol., 276, 307 (1997)
  16. Leslie , A.G.W. 1992 Recent changes to the MOSFLM package for processing film and image plate data. Joint CCP4 + ESF-EAMCB Newsletter on Protein Crystallography
  17. Bailey, Acta Crystallogr. D, 50, 760 (1994)
  18. Brunger, Acta Crystallogr. D, 54, 905 (1998)
  19. Navaza, Methods Enzymol., 276, 581 (1997)
  20. Jones, Acta Crystallogr. A., 47, 110 (1991)
  21. Lee, Biochemistry, 40, 7799 (2001)
  22. Guex, Electrophoresis, 18, 2714 (1997)
  23. Wallace, Protein Eng., 8, 127 (1995)
  24. DeLano, The PyMOL, Molecular Graphics System. (2002)
  25. Jefferey, Biochemistry, 39, 955 (2000)
  26. Read, J. Mol. Biol., 309, 447 (2001)
  27. Davies Christopher, Muirhead Hilary, Crystal structure of phosphoglucose isomerase from pig muscle and its complex with 5-phosphoarabinonate, 10.1002/prot.10255
  28. Cordeiro A.T, Godoi P.H.C, Silva C.H.T.P, Garratt R.C, Oliva G, Thiemann O.H, Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps, 10.1016/s1570-9639(02)00464-8
  29. Arsenieva, J. Mol. Biol., 323, 77 (2002)
Bibliographic reference Cordeiro, Artur T ; Michels, Paulus ; Delboni, Luiz F ; Thiemann, Otávio H. The crystal structure of glucose-6-phosphate isomerase from Leishmania mexicana reveals novel active site features.. In: European journal of biochemistry / FEBS, Vol. 271, no. 13, p. 2765-72 (2004)
Permanent URL http://hdl.handle.net/2078.1/26801