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Identification of a novel brain-specific and Reelin-regulated gene that encodes a protein colocalized with synapsin.

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Kuvbachieva, Anelia Bestel, Aude-Marie Tissir, Fadel [UCL] Maloum, Ismahane Guimiot, Fabien Goffinet, André [UCL] Simonneau, Michel

We carried out a screening of genes that are differentially expressed in normal mice and reeler mutants and are characterized by abnormal neuronal migration and neurite deployment due to defective Reelin signalling. A novel gene, provisionally named C61, was overexpressed in Reelin-deficient embryonic mouse brain RNA. C61 encodes a 3.7 kb mRNA that is brain specific and developmentally regulated, with predominant expression in differentiating neurons. The predicted protein is 664 amino acids long, and contains LAG1 and Ezrin/Radixin/Moesin-Myosin-Filament motifs, suggesting that it may function as an intracellular adaptor. From E14.5 to birth, C61 was highly expressed in all neuronal differentiation fields, with the highest signal in the telencephalic cortical plate and mitral cells in the olfactory bulb. When expressed as a GFP fusion protein in transfected non-neuronal cells and primary neurons, this protein localizes, respectively, to the nuclear membrane or axonal outgrowths, indicating a function in axonal traffic or signalling.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2004
Journal information "The European journal of neuroscience" - Vol. 20, no. 3, p. 603-610 (2004)
Peer reviewed yes
Publisher European Neuroscience Association ((United Kingdom) Oxford)
issn 0953-816X
e-issn 1460-9568
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Amino Acid Motifs - physiology ; Amino Acid Sequence ; Animals ; Animals, Newborn ; Blotting, Northern - methods ; Brain - embryology - growth & development - metabolism ; Caenorhabditis elegans ; Cell Adhesion Molecules, Neuronal - deficiency - genetics - metabolism - physiology ; Cell Line ; Cloning, Molecular ; Drosophila ; Embryo, Mammalian ; Embryo, Nonmammalian ; Extracellular Matrix Proteins - deficiency - genetics - physiology ; Gene Expression Regulation, Developmental ; Green Fluorescent Proteins ; Humans ; Immunohistochemistry - methods ; In Situ Hybridization - methods ; Luminescent Proteins - metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Neurologic Mutants ; Microtubule-Associated Proteins - metabolism ; Nerve Tissue Proteins ; Neurofibromin 2 - genetics - metabolism ; Neurons - metabolism ; Organ Specificity ; RNA, Messenger - biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Serine Endopeptidases ; Synapsins - metabolism ; Transfection ; Tubulin - metabolism ; Zebrafish
Keywords Axon ; Brain ; Differential display ; Neuron ; Reelin ; Synapse
Links
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Bibliographic reference Kuvbachieva, Anelia ; Bestel, Aude-Marie ; Tissir, Fadel ; Maloum, Ismahane ; Guimiot, Fabien ; et. al. Identification of a novel brain-specific and Reelin-regulated gene that encodes a protein colocalized with synapsin.. In: The European journal of neuroscience, Vol. 20, no. 3, p. 603-610 (2004)
Permanent URL http://hdl.handle.net/2078.1/25787