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Celecoxib after the onset of reperfusion reduces apoptosis in the amygdala.

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Kaloustian, Sévan Wann, Boubacar P Bah, Thierno M Falcao, Stéphanie Dufort, Anne-Marie Rousseau, Guy [UCL]

Reperfused myocardial infarction induces an inflammatory response that is responsible for local and systemic alterations. Among these, apoptosis observed in the amygdala following myocardial infarction has been pointed out as a consequence of such an inflammatory process. We hypothesized that inhibition of the inducible inflammatory enzyme Cox-2 during the reperfusion period may attenuate the apoptotic process in the amygdala. Anaesthetized rats were subjected to left anterior descending coronary artery occlusion for 40 min, followed by reperfusion. The Cox-2 antagonist Celecoxib (3 mg/kg i.p.) was administered 10 min after the onset of the reperfusion period. After 72 h of reperfusion, infarct size was determined and the lateral and medial amygdala were dissected from the brain. Infarct size was similar between untreated and Celecoxib-treated animals (40-45% of the area at risk). Cox-2 expression was significantly reduced in both parts of the amygdala in the Celecoxib group. Apoptosis regression was observed in the amygdala of the Celecoxib group as shown by decreased number of TUNEL positive cells and by decreased of caspase-3 activation. Bax/Bcl-2 ratio was not significantly altered by Celecoxib while Akt activation was increased in the lateral amygdala but not in the medial amygdala. This data indicates that inhibition of Cox-2 by Celecoxib is associated with regression of apoptosis in the amygdala following myocardial infarction.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2007
Journal information "Apoptosis : an international journal on programmed cell death" - Vol. 12, no. 11, p. 1945-51 (2007)
Peer reviewed yes
issn 1360-8185
Publication status Publié
Affiliation UCL
MESH Subject Amygdala - cytology - drug effects ; Animals ; Apoptosis - drug effects ; Cyclooxygenase Inhibitors - pharmacology ; Myocardial Reperfusion ; Myocardial Reperfusion Injury - drug therapy - prevention & control ; Pyrazoles - pharmacology ; Rats ; Rats, Sprague-Dawley ; Sulfonamides - pharmacology
Links
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Bibliographic reference Kaloustian, Sévan ; Wann, Boubacar P ; Bah, Thierno M ; Falcao, Stéphanie ; Dufort, Anne-Marie ; et. al. Celecoxib after the onset of reperfusion reduces apoptosis in the amygdala.. In: Apoptosis : an international journal on programmed cell death, Vol. 12, no. 11, p. 1945-51 (2007)
Permanent URL http://hdl.handle.net/2078.1/25623