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Lack of SC/pIgR-mediated epithelial transport of a human polymeric IgA devoid of J chain: in vitro and in vivo studies.

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Vaerman, Jean-Pierre [UCL] Langendries, Agnès [UCL] Giffroy, D Brandtzaeg, P. Kobayashi, K.

Three human polymeric IgA (pIgA) myeloma proteins of tetrameric size were compared for their J-chain content, their in vitro secretory component (SC)-binding ability, and their capacity to be transcytosed by polymeric immunoglobulin receptor (pIgR)-expressing epithelial cells in vitro and rat hepatocytes in vivo. One of the three pIgA preparations, pIgA-L, was shown to lack J chain and was unable to combine with purified free human and rat SC, whereas pIgA-G and pIgA-C contained J chain and combined readily with SC. Furthermore, pIgA-L was not transferred into rat bile after intravenous injection, and was hardly transported apically by polarized Madin-Darbey canine kidney cell monolayers expressing the human pIgR, whereas pIgA-G and pIgA-C were efficiently transported in both test systems. Together with our recent demonstration that antibodies to human J chain block the SC/pIgR-mediated epithelial transport of pIgA, these data unanimously confirm the proposed key role of J chain in the epithelial transport of polymeric immunoglobulins into exocrine secretions.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 1998
Journal information "Immunology" - Vol. 95, no. 1, p. 90-6 (1998)
Peer reviewed yes
issn 0019-2805
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Animals ; Bile - immunology - metabolism ; Biological Transport ; Cells, Cultured ; Dogs ; Electrophoresis ; Epithelial Cells - metabolism ; Humans ; Immunoelectrophoresis ; Immunoglobulin A ; Immunoglobulin J-Chains ; Infant, Newborn ; Multiple Myeloma - immunology - metabolism ; Myeloma Proteins - immunology - isolation & purification - metabolism ; Protein Binding ; Rats ; Receptors, Immunologic - metabolism ; Receptors, Polymeric Immunoglobulin - metabolism ; Secretory Component - metabolism ; Ultracentrifugation
Links
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Bibliographic reference Vaerman, Jean-Pierre ; Langendries, Agnès ; Giffroy, D ; Brandtzaeg, P. ; Kobayashi, K.. Lack of SC/pIgR-mediated epithelial transport of a human polymeric IgA devoid of J chain: in vitro and in vivo studies.. In: Immunology, Vol. 95, no. 1, p. 90-6 (1998)
Permanent URL http://hdl.handle.net/2078.1/24182