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Insulin antagonizes ischemia-induced Thr172 phosphorylation of AMP-activated protein kinase alpha-subunits in heart via hierarchical phosphorylation of Ser485/491.

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Horman, Sandrine [UCL] Vertommen, Didier [UCL] Heath, Richard Neumann, Dietbert Mouton, Véronique [UCL] Hue, Louis [UCL] Rider, Mark H. [UCL]

Previous studies showed that insulin antagonizes AMP-activated protein kinase activation by ischemia and that protein kinase B might be implicated. Here we investigated whether the direct phosphorylation of AMP-activated protein kinase by protein kinase B might participate in this effect. Protein kinase B phosphorylated recombinant bacterially expressed AMP-activated protein kinase heterotrimers at Ser(485) of the alpha1-subunits. In perfused rat hearts, phosphorylation of the alpha1/alpha2 AMP-activated protein kinase subunits on Ser(485)/Ser(491) was increased by insulin and insulin pretreatment decreased the phosphorylation of the alpha-subunits at Thr(172) in a subsequent ischemic episode. It is proposed that the effect of insulin to antagonize AMP-activated protein kinase activation involves a hierarchical mechanism whereby Ser(485)/Ser(491) phosphorylation by protein kinase B reduces subsequent phosphorylation of Thr(172) by LKB1 and the resulting activation of AMP-activated protein kinase.

Document type Article de périodique (Journal article) – In Vitro, Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2006
Journal information "The Journal of biological chemistry" - Vol. 281, no. 9, p. 5335-40 (2006)
Peer reviewed yes
issn 0021-9258
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject AMP-Activated Protein Kinases ; Adenosine Monophosphate - metabolism ; Animals ; Enzyme Activation ; Insulin - metabolism ; Male ; Multienzyme Complexes - chemistry - genetics - metabolism ; Myocardial Ischemia ; Myocardium - enzymology ; Phosphorylation ; Protein Subunits - genetics - metabolism ; Protein-Serine-Threonine Kinases - chemistry - genetics - metabolism ; Proto-Oncogene Proteins c-akt - genetics - metabolism ; Rats ; Rats, Wistar ; Serine - metabolism ; Threonine - metabolism
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Bibliographic reference Horman, Sandrine ; Vertommen, Didier ; Heath, Richard ; Neumann, Dietbert ; Mouton, Véronique ; et. al. Insulin antagonizes ischemia-induced Thr172 phosphorylation of AMP-activated protein kinase alpha-subunits in heart via hierarchical phosphorylation of Ser485/491.. In: The Journal of biological chemistry, Vol. 281, no. 9, p. 5335-40 (2006)
Permanent URL http://hdl.handle.net/2078.1/22000