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Towards melanin radicals detection in melanomas by electron paramagnetic resonance (EPR) spectroscopy: a proof-of-concept study.

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Desmet, Céline M [UCL] Danhier, Pierre [UCL] Acciardo, Stefania [UCL] Levêque, Philippe [UCL] Gallez, Bernard [UCL]

Melanoma is the most aggressive skin tumour type. Although complete cure can be achieved when the whole tumour is resected, prognostic dramatically drops when melanoma cells reach deeper tissues and lymph nodes. Hence, there is an urgent need to develop accurate tools allowing () discriminating benign naevi from malignant tumours and () being able to characterise melanoma infiltration. For that purpose, we exploited the paramagnetic properties of melanin by using electron paramagnetic resonance (EPR) spectroscopy to measure the melanin content in pigmented (B16F10 cancer cells) and non-pigmented melanomas (WM2664 cancer cells) inoculated intradermally in nude mice. Specifically, we took advantage of a new clinical EPR device (1 GHz), which provides sensitive measurements of radical species . Results showed that the melanin-specific EPR signal increased with tumour growth in pigmented tumours, whereas no EPR signal could be detected in achromic melanomas. These data plead for the development of new EPR spectrometers/imagers with an improved in-depth resolution for the detection of invasive melanomas.

Document type Article de périodique (Journal article)
Access type Accès libre
Publication date 2019
Language Anglais
Journal information "Free radical research" - Vol. 53, no.4, p. 405-410 (2019)
Peer reviewed yes
e-issn 1029-2470
Publication status Publié
Affiliation UCL - SSS/LDRI - Louvain Drug Research Institute
Keywords EPR ; ESR ; melanin ; melanoma
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Bibliographic reference Desmet, Céline M ; Danhier, Pierre ; Acciardo, Stefania ; Levêque, Philippe ; Gallez, Bernard. Towards melanin radicals detection in melanomas by electron paramagnetic resonance (EPR) spectroscopy: a proof-of-concept study.. In: Free radical research, Vol. 53, no.4, p. 405-410 (2019)
Permanent URL http://hdl.handle.net/2078.1/217342