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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

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Théry, Clotilde Witwer, Kenneth W Aikawa, Elena Alcaraz, Maria Jose Anderson, Johnathon D Demaret, Tanguy [UCL] Lombard, Catherine [UCL] Mullier, François [UCL]

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, andmany other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès interdit
Publication date 2018
Language Anglais
Journal information "Journal of Extracellular Vesicles" - Vol. 7, no. 1, p. 1535750 [1-43] (2018)
Peer reviewed yes
Publisher Taylor & Francis ((United States) Philadelphia, PA)
e-issn 2001-3078
Publication status Publié
Affiliations UCL - SSS/IREC/MONT - Pôle Mont Godinne
UCL - SSS/IREC/PEDI - Pôle de Pédiatrie
UCL - (MGD) Laboratoire de biologie clinique
UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique
Keywords Extracellular vesicles ; Exosomes ; Ectosomes ; Microvesicles ; Minimal information requirement ; Guidelines ; Standardization ; Microparticles ; Rigor
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Permanent URL http://hdl.handle.net/2078.1/208114