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Activation of plant plasma membrane H+-ATPase by 14-3-3 proteins is negatively controlled by two phosphorylation sites within the H+-ATPase C-terminal region.

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Duby, Geoffrey [UCL] Poreba, Wojciech Piotrowiak, Dominik [UCL] Bobik, Krzysztof [UCL] Derua, Rita Boutry, Marc [UCL]

The proton pump ATPase (H+-ATPase) of the plant plasma membrane is regulated by an auto-inhibitory C-terminal domain, which can be displaced by phosphorylation of the penultimate Thr residue and the subsequent binding of 14-3-3 proteins. We performed a mass spectrometric analysis of plasma membrane H+-ATPase isoform 2 (PMA2) isolated from Nicotiana tabacum suspension cells and identified two new phosphorylated residues in the enzyme 14-3-3 protein binding site: Thr931 and Ser938. When PMA2 was expressed in Saccharomyces cerevisiae, mutagenesis of each of these two residues into Asp prevented growth of a yeast strain devoid of its own H+-ATPases. When the Asp mutations were individually introduced in a constitutively activated mutant of PMA2 (Glu14Asp), they still allowed yeast growth, but at a reduced rate. Purification of His-tagged PMA2 showed that the Thr931Asp or Ser938Asp mutation prevented 14-3-3 protein binding, although the penultimate Thr955 was still phosphorylated, indicating that Thr955 phosphorylation is not sufficient for full enzyme activation. Expression of PMA2 in an N. tabacum cell line also showed absence of 14-3-3 protein binding resulting from the Thr931Asp or Ser938Asp mutation. Together, the data show that activation of H+-ATPase by the binding of 14-3-3 proteins is negatively controlled by phosphorylation of two residues in the H+-ATPase 14-3-3 protein binding site. The data also show that phosphorylation of the penultimate Thr and 14-3-3 binding each contribute in part to H+-ATPase activation.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2009
Language Anglais
Journal information "The Journal of biological chemistry" - Vol. 284, no. 7, p. 4213-21 (2009)
Peer reviewed yes
issn 0021-9258
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Tobacco - enzymology - genetics ; Saccharomyces cerevisiae - genetics ; Recombinant Proteins - genetics - metabolism ; Proton-Translocating ATPases - genetics - metabolism ; Plant Proteins - genetics - metabolism ; Phosphorylation - physiology ; Mutagenesis ; Mass Spectrometry ; Gene Expression ; Enzyme Activation - genetics ; Amino Acid Substitution ; 14-3-3 Proteins - genetics - metabolism
Links
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Bibliographic reference Duby, Geoffrey ; Poreba, Wojciech ; Piotrowiak, Dominik ; Bobik, Krzysztof ; Derua, Rita ; et. al. Activation of plant plasma membrane H+-ATPase by 14-3-3 proteins is negatively controlled by two phosphorylation sites within the H+-ATPase C-terminal region.. In: The Journal of biological chemistry, Vol. 284, no. 7, p. 4213-21 (2009)
Permanent URL http://hdl.handle.net/2078.1/20742