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Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

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De Berdt, Pauline [UCL] Bottemanne, Pauline [UCL] Bianco, John [UCL] Al Houayek, Mireille [UCL] Diogenes, Anibal Muccioli, Giulio [UCL] des Rieux, Anne [UCL]

Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2018
Language Anglais
Journal information "Cellular and Molecular Life Sciences" - Vol. 75, p. 2843-2856 (2018)
Peer reviewed yes
Publisher Springer Nature
issn 1420-682X
e-issn 1420-9071
Publication status Publié
Affiliation UCL - SSS/LDRI - Louvain Drug Research Institute
Keywords Dental stem cells ; Differentiation ; Inflammation ; Oligodendrocyte progenitor cells ; Spinal cord
Links
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Bibliographic reference De Berdt, Pauline ; Bottemanne, Pauline ; Bianco, John ; Al Houayek, Mireille ; Diogenes, Anibal ; et. al. Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion. In: Cellular and Molecular Life Sciences, Vol. 75, p. 2843-2856 (2018)
Permanent URL http://hdl.handle.net/2078.1/199631