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Contribution of plasma membrane lipid domains to red blood cell (re)shaping.

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Léonard, Catherine [UCL] Conrard, Louise [UCL] Guthmann, Manuel [UCL] Pollet, Hélène [UCL] Carquin, Mélanie [UCL] Vermylen, Christiane [UCL] Gailly, Philippe [UCL] Van Der Smissen, Patrick [UCL] Mingeot-Leclercq, Marie-Paule [UCL] Tyteca, Donatienne [UCL]

Although lipid domains have been evidenced in several living cell plasma membranes, their roles remain largely unclear. We here investigated whether they could contribute to function-associated cell (re)shaping. To address this question, we used erythrocytes as cellular model since they (i) exhibit a specific biconcave shape, allowing for reversible deformation in blood circulation, which is lost by membrane vesiculation upon aging; and (ii) display at their outer plasma membrane leaflet two types of submicrometric domains differently enriched in cholesterol and sphingomyelin. We here reveal the specific association of cholesterol- and sphingomyelin-enriched domains with distinct curvature areas of the erythrocyte biconcave membrane. Upon erythrocyte deformation, cholesterol-enriched domains gathered in high curvature areas. In contrast, sphingomyelin-enriched domains increased in abundance upon calcium efflux during shape restoration. Upon erythrocyte storage at 4 °C (to mimick aging), lipid domains appeared as specific vesiculation sites. Altogether, our data indicate that lipid domains could contribute to erythrocyte function-associated (re)shaping.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2017
Language Anglais
Journal information "Scientific Reports" - Vol. 7, no. 1, p. 4264 [1-17] (2017)
Peer reviewed yes
Publisher Nature Publishing Group ((United Kingdom) London)
e-issn 2045-2322
Publication status Publié
Affiliations UCL - SSS/DDUV/CELL - Biologie cellulaire
UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire
UCL - SSS/IREC/PEDI - Pôle de Pédiatrie
UCL - SSS/LDRI - Louvain Drug Research Institute
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Bibliographic reference Léonard, Catherine ; Conrard, Louise ; Guthmann, Manuel ; Pollet, Hélène ; Carquin, Mélanie ; et. al. Contribution of plasma membrane lipid domains to red blood cell (re)shaping.. In: Scientific Reports, Vol. 7, no. 1, p. 4264 [1-17] (2017)
Permanent URL http://hdl.handle.net/2078.1/185954