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Regulation of mesenchymal stem cell 3D microenvironment: From macro to microfluidic bioreactors

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Sart, Sébastien Agathos, Spiros N. [UCL] Li, Yan Ma, Teng

Human mesenchymal stem cells (hMSCs) have emerged as an important cell type in cell therapy and tissue engineering. In these applications, maintaining the therapeutic properties of hMSCs requires tight control of the culture environments and the structural cell organizations. Bioreactor systems are essential tools to achieve these goals in the clinical-scale expansion and tissue engineering applications. This review summarizes how different bioreactors provide cues to regulate the structure and the chemico-mechanical microenvironment of hMSCs with a focus on 3D organization. In addition to conventional bioreactors, recent advances in microfluidic bioreactors as a novel approach to better control the hMSC microenvironment are also discussed. These advancements highlight the key role of bioreactor systems in preserving hMSC’s functional properties by providing dynamic and temporal regulation of in vitro cellular microenvironment.

Document type Article de périodique (Journal article) – Synthèse de littérature
Access type Accès libre
Publication date 2016
Language Anglais
Journal information "Biotechnology Journal" - Vol. 11, no.1, p. 43-57 (2016)
Peer reviewed yes
Publisher Wiley - V C H Verlag GmbH & Co. KGaA ((Germany) Weinheim)
issn 1860-6768
e-issn 1860-7314
Publication status Publié
Affiliations UCL - SST/ELI/ELIM - Applied Microbiology
UCL - SST/ISV - Institut des sciences de la vie
Florida State University - Department of Chemical & Biomedical Engineering
Ecole Polytechnique, Palaiseau, France - Hydrodynamics Laboratory, CNRS UMR 7646
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Keywords Bioreactors ; Mesenchymal stem cells ; Microenvironment ; Microfluidics ; Three-dimensional cell culture
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