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Rhizophagus irregularis MUCL 41833 can colonize and improve P uptake of Plantago lanceolata after exposure to ionizing gamma radiation in root organ culture

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Kothamasi, David [Belgian Nuclear Research Center, Mol, Belgium] Wannijn, Jean [Belgian Nuclear Research Center, Mol, Belgium] van Hees, May [Belgian Nuclear Research Center, Mol, Belgium] Nauts, Robin [Belgian Nuclear Research Center, Mol, Belgium] van Gompel, Axel [Belgian Nuclear Research Center, Mol, Belgium] Cranenbrouck, Sylvie [UCL] Declerck, Stephan [UCL] Vandenhove, Hildegarde [Belgian Nuclear Research Center, Mol, Belgium]

Long-lived radionuclides such as 90Sr and 137Cs can be naturally or accidentally deposited in the upper soil layers where they emit β/γ radiation. Previous studies have shown that arbuscular mycorrhizal fungi (AMF) can accumulate and transfer radionuclides from soil to plant, but there have been no studies on the direct impact of ionizing radiation on AMF. In this study, root organ cultures of the AMF Rhizophagus irregularis MUCL 41833 were exposed to 15.37, 30.35, and 113.03 Gy gamma radiation from a 137Cs source. Exposed spores were subsequently inoculated to Plantago lanceolata seedlings in pots, and root colonization and P uptake evaluated. P. lanceolata seedlings inoculated with non-irradiated AMF spores or with spores irradiated with up to 30.35 Gy gamma radiation had similar levels of root colonization. Spores irradiated with 113.03 Gy gamma radiation failed to colonize P. lanceolata roots. P content of plants inoculated with non-irradiated spores or of plants inoculated with spores irradiated with up to 30.35 Gy gamma radiation was higher than in non-mycorrhizal plants or plants inoculated with spores irradiated with 113.03 Gy gamma radiation. These results demonstrate that spores of R. irregularis MUCL 41833 are tolerant to chronic ionizing radiation at high doses.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2016
Language Anglais
Journal information "Mycorrhiza" - Vol. 26, no.3, p. 257-262 (2016)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 0940-6360
e-issn 1432-1890
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Rhizophagus irregularis ; Spores ; Gamma radiation ; Plantago lanceolata ; Root colonization ; Plant P uptake ; 3145
Links
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Bibliographic reference Kothamasi, David ; Wannijn, Jean ; van Hees, May ; Nauts, Robin ; van Gompel, Axel ; et. al. Rhizophagus irregularis MUCL 41833 can colonize and improve P uptake of Plantago lanceolata after exposure to ionizing gamma radiation in root organ culture. In: Mycorrhiza, Vol. 26, no.3, p. 257-262 (2016)
Permanent URL http://hdl.handle.net/2078.1/180926