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ISPD produces CDP-ribitol used by FKTN and FKRP to transfer ribitol phosphate onto α-dystroglycan

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Gerin, Isabelle [UCL] Ury, Benoît [UCL] Breloy, Isabelle Bouchet-Seraphin, Céline Bolsée, Jennifer [UCL] Halbout, Mathias [UCL] Graff, Julie [UCL] Vertommen, Didier [UCL] Muccioli, Giulio [UCL] Grahn, Ammi [UCL] Van Schaftingen, Emile [UCL] Bommer, Guido [UCL]

Mutations in genes required for the glycosylation of α-dystroglycan lead to muscle and brain diseases known as dystroglycanopathies. However, the precise structure and biogenesis of the assembled glycan are not completely understood. Here we report that three enzymes mutated in dystroglycanopathies can collaborate to attach ribitol phosphate onto α-dystroglycan. Specifically, we demonstrate that isoprenoid synthase domain-containing protein (ISPD) synthesizes CDP-ribitol, present in muscle, and that both recombinant fukutin (FKTN) and fukutin-related protein (FKRP) can transfer a ribitol phosphate group from CDP-ribitol to α-dystroglycan. We also show that ISPD and FKTN are essential for the incorporation of ribitol into α-dystroglycan in HEK293 cells. Glycosylation of α-dystroglycan in fibroblasts from patients with hypomorphic ISPD mutations is reduced. We observe that in some cases glycosylation can be partially restored by addition of ribitol to the culture medium, suggesting that dietary supplementation with ribitol should be evaluated as a therapy for patients with ISPD mutations.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2016
Language Anglais
Journal information "Nature Communications" - Vol. 7, no. 1, p. 11534 [1-15] (2016)
Peer reviewed yes
Publisher Nature Publishing Group ((United Kingdom) London)
e-issn 2041-1723
Publication status Publié
Affiliations UCL - SSS/DDUV - Institut de Duve
UCL - SSS/LDRI - Louvain Drug Research Institute
Links
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Bibliographic reference Gerin, Isabelle ; Ury, Benoît ; Breloy, Isabelle ; Bouchet-Seraphin, Céline ; Bolsée, Jennifer ; et. al. ISPD produces CDP-ribitol used by FKTN and FKRP to transfer ribitol phosphate onto α-dystroglycan. In: Nature Communications, Vol. 7, no. 1, p. 11534 [1-15] (2016)
Permanent URL http://hdl.handle.net/2078.1/175322