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Clinical potential of electroporation for gene therapy and DNA vaccine delivery.

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Lambricht, Laure [UCL] Lopes, Alessandra [UCL] Kos, Spela Sersa, Gregor Préat, Véronique [UCL] Vandermeulen, Gaëlle [UCL]

(eng) INTRODUCTION: Electroporation allows efficient delivery of DNA into cells and tissues, thereby improving the expression of therapeutic or immunogenic proteins that are encoded by plasmid DNA. This simple and versatile method holds a great potential and could address unmet medical needs such as the prevention or treatment of many cancers or infectious diseases. AREAS COVERED: This review explores the electroporation mechanism and the parameters affecting its efficacy. An analysis of past and current clinical trials focused on DNA electroporation is presented. The pathologies addressed, the protocol used, the treatment outcome and the tolerability are highlighted. In addition, several of the possible optimization strategies for improving patient compliance and therapeutic efficacy are discussed such as plasmid design, use of genetic adjuvants for DNA vaccines, choice of appropriate delivery site and electrodes as well as pulse parameters. EXPERT OPINION: The growing number of clinical trials and the results already available underline the strong potential of DNA electroporation which combines both safety and efficiency. Nevertheless, it remains critical to further increase fundamental knowledge to refine future strategies, to develop concerted and common DNA electroporation protocols and to continue exploring new electroporation-based therapeutic options.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2016
Language Anglais
Journal information "Expert Opinion on Drug Delivery" - Vol. 13, no. 2, p. 295-310 (2016)
Peer reviewed yes
Publisher Informa Healthcare ((United Kingdom) London)
issn 1742-5247
e-issn 1744-7593
Publication status Publié
Affiliation UCL - SSS/LDRI - Louvain Drug Research Institute
Keywords Clinical trials ; DNA vaccines ; Electroporation ; Gene delivery ; Gene electrotransfer ; Gene therapy ; Genetic adjuvants
Links
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Bibliographic reference Lambricht, Laure ; Lopes, Alessandra ; Kos, Spela ; Sersa, Gregor ; Préat, Véronique ; et. al. Clinical potential of electroporation for gene therapy and DNA vaccine delivery.. In: Expert Opinion on Drug Delivery, Vol. 13, no. 2, p. 295-310 (2016)
Permanent URL http://hdl.handle.net/2078.1/171506