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Staggered multi-site low-frequency electrostimulation effectively induces locomotor patterns in the isolated rat spinal cord

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Dose, Francesco Deumens, Ronald [UCL] Forget, Patrice [UCL] Taccola, Giuliano

(eng) STUDY DESIGN: Experimental animal study. OBJECTIVES: Epidural stimulation has been used to activate locomotor patterns after spinal injury and typically employs synchronous trains of high-frequency stimuli delivered directly to the dorsal cord, thereby recruiting multiple afferent nerve roots. Here we investigate how spinal locomotor networks integrate multi-site afferent input and address whether frequency coding is more important than amplitude to activate locomotor patterns. SETTING: Italy and Belgium. METHODS: To investigate the importance of input intensity and frequency in eliciting locomotor activity, we used isolated neonatal rat spinal cords to record episodes of fictive locomotion (FL) induced by electrical stimulation of single and multiple dorsal roots (DRs), employing different stimulating protocols. RESULTS: FL was efficiently induced through staggered delivery (delays 0.5 to 2 s) of low-frequency pulse trains (0.33 and 0.67 Hz) to three DRs at intensities sufficient to activate ventral root reflexes. Delivery of the same trains to a single DR or synchronously to multiple DRs remained ineffective. Multi-site staggered trains were more efficient than randomized pulse delivery. Weak trains simultaneously delivered to DRs failed to elicit FL. Locomotor rhythm resetting occurred with single pulses applied to various distant DRs. CONCLUSION: Electrical stimulation recruited spinal networks that generate locomotor programs when pulses were delivered to multiple sites at low frequency. This finding might help devising new protocols to optimize the increasingly more common use of epidural implantable arrays to treat spinal dysfunctions.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2016
Language Anglais
Journal information "Spinal Cord" - Vol. 54, no. 2, p. 93-101 (2016)
Peer reviewed yes
Publisher Nature Publishing Group ((United Kingdom) Houndmills)
issn 1362-4393
e-issn 1476-5624
Publication status Publié
Affiliations UCL - (SLuc) Service d'anesthésiologie
UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire
Links
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Bibliographic reference Dose, Francesco ; Deumens, Ronald ; Forget, Patrice ; Taccola, Giuliano. Staggered multi-site low-frequency electrostimulation effectively induces locomotor patterns in the isolated rat spinal cord. In: Spinal Cord, Vol. 54, no. 2, p. 93-101 (2016)
Permanent URL http://hdl.handle.net/2078.1/168236