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Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria

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Alsteens, David [UCL] Trabelsi, Heykel [UCL] Soumillion, Patrice [UCL] Dufrêne, Yves [UCL]

Force-distance (FD) curve-based atomic force microscopy is a valuable tool to simultaneously image the structure and map the biophysical properties of biological samples at the nanoscale. Traditionally, FD-based atomic force microscopy has been severely limited by its poor temporal and lateral resolutions. Here we report the use of advanced FD-based technology combined with biochemically sensitive tips to image filamentous bacteriophages extruding from living bacteria at unprecedented speed and resolution. Directly correlated multiparametric images of the structure, adhesion and elasticity of infected bacteria demonstrate that the sites of assembly and extrusion localize at the bacterial septum in the form of soft nanodomains surrounded by stiff cell wall material. The quantitative nano-bio-imaging method presented here offers a wealth of opportunities for mapping the physical properties and molecular interactions of complex biosystems, from viruses to tissues.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2013
Language Anglais
Journal information "Nature Communications" - Vol. 4, p. 1-7 (2013)
Peer reviewed yes
Publisher Nature Publishing Group ((United Kingdom) London)
issn 2041-1723
e-issn 2041-1723
Publication status Publié
Affiliations UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Links
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Bibliographic reference Alsteens, David ; Trabelsi, Heykel ; Soumillion, Patrice ; Dufrêne, Yves. Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria. In: Nature Communications, Vol. 4, p. 1-7 (2013)
Permanent URL http://hdl.handle.net/2078.1/135929