User menu

Thymic nurse cells in culture: morphological and antigenic characterization.

Bibliographic reference Toussaint-Demylle, D ; Scheiff, Jean-Marie ; Haumont, Stanislas. Thymic nurse cells in culture: morphological and antigenic characterization.. In: Cell and tissue research, Vol. 272, no. 2, p. 343-54 (1993)
Permanent URL
  1. Ambesi-Impiombato FS, Parks LAM, Coon HG (1980) Cutlure of hormone-dependent functional epithelial cells from rat thyroids. Proc Natl Acad Sci USA 77:3455?3459
  2. Andrews P, Boyd RL (1985) The murine thymic nurse cell: an isolated thymic microenvironment. Eur J Immunol 15:36?42
  3. Barnes D, Sato G (1980) Serum-free cell culture: a unifying approach. Cell 22:649?655
  4. Berridge MV, Nargang JC, Loverde PT, Golub ES (1980) The culture of mouse thymic reticulum cells: the effect of homologous serum on the establishment of primary cultures. J Immunol 124:2738?2746
  5. Berrih S, Arenzana-Seisdedos F, Cohen S, Devos R, Charron D, Virelizier JL (1985) Interferon-? modulates HLA class II antigen expression on cultured human thymic epithelial cells. J Immunol 135:1165?1171
  6. Bertolero F, Kaighn ME, Camalier RF, Saffiotti U (1986) Effects of serum and serum-derived factors on growth and differentiation of mouse keratinocytes. In Vitro 22:423?428
  7. Bhattacharya A, Dorf ME, Springer TA (1981) A shared alloantigenic determinant on Ia antiens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol 127:2488?2495
  8. Boyce ST, Ham RG (1983) Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol 81:33s-40s
  9. Boyd RL, Oberhuber G, Hala K, Wick G (1984) Obese strain (OS) chickens with spontaneous autoimmune thyroiditis have a deficiency in thymic nurse cells J Immunol 132:718?724
  10. Davison PM, Bensch K, Karasek MA (1983) Isolation and longterm serial cultivation of endothelial cells from the microvessels of the adult human dermis. In Vitro 19:937?945
  11. DeLuca D, Mizel SB (1986) I-A-positive nonlymphoid cells and T cell development in murine fetal thymus organ cultures: interleukin 1 circumvents the block in T cell differentiation induced by monoclonal anti-I-A antibodies. J Immunol 137:1435?1441
  12. Denning SM, Tuck DT, Singer KH, Haynes BF (1987) Human thymic epithelial cells function as accessory cells for autologous mature thymocyte activation. J Immunol 138:680?686
  13. Dijkmans R, Volckaert G, Van Damme J, De Ley M, Billiau A, De Somer P (1985) Molecular cloning of murine interferon gamma (MuIFN-?) cDNA and its expression in heterologous mammalian cells. J Interferon Res 5:511?520
  14. Eagle H (1959) Amino acid metabolism in mammalian cell cultures. Science 130:432?437
  15. Ehmann UK, Shiurba RA, Peterson WD (1986) Long-term proliferation of mouse thymic epithelial cells in culture. In Vitro 22:738?748
  16. Ewijk W van (1984) Immunohistology of lymphoid and non-lymphoid cells in the thymus in relation to T lymphocyte differentiation. Am J Anat 170:311?330
  17. Ewijk W van, Rouse RV, Weissman IL (1980) Distribution of H-2 microenvironments in the mouse thymus. Immunoelectron microscopic identification of I-A and H-2K bearing cells. J Histochem Cytochem 28:1089?1099
  18. Freshney RI (1986) Introduction: principles of sterile technique and cell propagation. In: Freshney RI (ed) Animal cell culture, a practical approach. IRL Press, Oxford, pp 1?11
  19. Gallily R, Zeira M, Stain I (1985) Thymus-derived macrophages in long-term culture: release of IL-1, stimulation of MLR and expression of tumoricidal activity. Immunology 55:165?172
  20. Garland JM, Dexter TM (1981) Studies on the in vitro culture of murine fetal thymus: effect of factors promoting epithelial growth. Am J Reprod Immunol 1:129?139
  21. Gilbert SF, Migeon BR (1975) D-valine as a selective agent for normal human and rodent epithelial cells in culture. Cell 5:11?17
  22. Ham RG (1983) Growth of normal human cells in defined media. In: Fischer G, Wieser RJ (eds) Hormonally defined media. Springer, Berlin Heidelberg New York, pp 16?30
  23. Hennings H, Holbrook KA, Yuspa SH (1983) Potassium mediation of calcium-induced terminal differentiation of epidermal cells in culture. J Invest Dermatol 81:50s-54s
  24. Jason JM, Janeway CA (1984) In vitro cultivation of nonlymphoid thymic cells: morphological and immunological characterization. Clin Immunol Immunopathol 30:214?226
  25. Kaji K, Matsuo M (1981) Aging of chick embryo fibroblasts in vitro. V. Time course studies on polyploid nucleus accumulation. Exp Cell Res 131:410?412
  26. Kendall MD, Schuurman HJ, Fenton J, Broekhuizen R, Kampinga J (1988) Implantation of cultured thymic fragments in congenitally athymic (nude) rats. Ultrastructural characteristics of the developing microenvironment. Cell Tissue Res 254:283?294
  27. Kyewski BA, Kaplan HS (1982) Lymphoepithelial interactions in the mouse thymus: phenotypic and kinetic studies on thymic nurse cells. J Immunol 128:2287?2294
  28. Ledbetter JA, Herzenberg LA (1979) Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev 47:63?90
  29. Leenen PJM Melis ML, Ewijk W van (1987) Single-cell immuno-?-galactosidase staining of heterogeneous populations. Practical application on limited cell numbers. Histochem J 19:497?503
  30. Lo D, Sprent J (1986) Exogenous control of I-A expression in fetal thymus explants. J Immunol 137:1772?1775
  31. Lobach DF, Itoh T, Singer LH, Haynes BF (1987) The thymic microenvironment. Characterization of in vitro differentiation of the IT26R21 rat thymic epithelial cell line. Differentiation 34:50?59
  32. Loor F (1979) Mouse thymus reticulo-epithelial (RE) cells in vitro: isolation cultivation and preliminary characterization. Immunology 37:157?177
  33. Mandel T (1970) Differentiation of epithelial cells in the mouse thymus. Z Zellforsch 106:498?515
  34. Mandel T, Russell PJ (1971) Differentiation of foetal mouse thymus. Ultrastructure of organ cultures and of subcapsular grafts. Immunology 21:659?674
  35. Masuda A, Amo H, Matsuyama M (1984) Cytological characteristics of cultured epithelial cells from thymomas in BUF/Mna rats. Virchows Arch [A] 46:283?296
  36. Masuda A, Nishimoto Y, Morita T, Matsuyama M (1985) Dexamethasone-induced changes in morphology and keratin organization of rat thymic epithelial cells in primary cultures. Exp Cell Res 160:343?355
  37. Matsumoto Y, Furuya A, Kobayashi T, Tsukagoshi H (1986) Primary cultures of human myasthenia gravis thymus and normal thymus. Studies of cell morphology, cell proliferative pattern and localization of ?-bungarotoxin binding sites on cultured thymic cells. J Neurol Sci 75:121?133
  38. Maurer HR (1986) Towards chemically-defined, serum-free media for mammalian cell culture. In: Freshney RI (ed) Animal cell culture, a practical approach. IRL Press, Oxford, pp 13?31
  39. Mitsui Y, Schneider EL (1976) Increased nuclear, sizes in senescent human diploid fibroblast cultures. Exp Cell Res 100:147?152
  40. Mizutani S, Watt SM, Robertson D, Hussein US, Healy LE, Furley AJW, Greaves MF (1987) Cloning of human thymic subcapsular cortex epithelial cells with T-lymphocyte binding sites and hemopoietic growth factor activity. Proc Natl Acad Sci USA 84:4999?5003
  41. Mukherjee AB, Weinstein ME (1986) Culture media variation as related to in vitro aging of human fibroblasts: I. Effects on population doubling, nuclear volume and nuclear morphology. Mech Age Dev 37:55?67
  42. Nabarra B, Andrianarison I (1987) Pattern of secretion in thymic epithelial cells: ultrastructural studies of the effect of blockage at various levels. Cell Tissue Res 249:171?178
  43. Nieburgs AC, Picciano PT, Korn JH, McCalister T, Allred C, Cohen S (1985) In vitro growth and maintenance of two morphologically distinct populations of thymic epithelial cells. Cell Immunol 90:439?450
  44. Nieburgs AC, Korn JH, Picciano P, Cohen S (1987) Thymic epithelium in vitro. IV. Regulation of growth and mediator production by epidermal growth factor. Cell Immunol 108:396?404
  45. O'Donnell-Tormey J, Nathan CF, Lanks K, DeBoer CJ, Harpe J de la (1987) Secretion of pyruvate. An antioxidant defense of mammalian cells. J Exp Med 165:500?514
  46. Papiernik M, Homo-Delarche F (1983) Thymic reticulum in mice. III. Phagocytic cells of the thymic reticulum in culture secrete both prostaglandin E2 and interleukin 1 which regulate thymocyte proliferation. Eur J Immunol 13:689?692
  47. Papiernik M, Nabarra B (1981) Thymic reticulum in mice. I. Cellular ultrastructure in vitro and functional role. Thymus 3:345?358
  48. Papiernik M, Nabarra B, Bach JF (1975) In vitro culture of functional human thymic epithelium. Clin Exp Immunol 19:281?287
  49. Pereira G, Clermont Y (1971) Distribution of cell web-containing epithelial reticular cells in the rat thymus. Anat Rec 169:613?626
  50. Phillips HJ (1973) Dye exclusion tests for cell viability. In: Kruse PF, Patterson MK (eds) Tissue culture, methods and applications. Academic Press, New York London, pp 406?408
  51. Piltch A, Naylor P, Hayashi J (1988) A cloned rat thymic epithelial cell line established from serum-free selective culture. In Vitro 24:289?293
  52. Raedler A, Arndt R, Thiele HG (1979) Evidence for the expression of Thy-1-analogous structures on human thymic epithelial cells. Scand J Immunol 10:69?76
  53. Ransom J, Fischer M, Mercer L, Zlotnik A (1987a) Lymphokinemediated induction of antigen-presenting ability in thymic stromal cells. J Immunol 139:2620?2628
  54. Ransom J, Fischer M, Mosmann T, Yokota T, DeLuca D, Schumacher J, Zlotnik A (1987b) Interferon-? is produced by activated immature mouse thymocytes and inhibits the interleukin 4-induced proliferation of immature thymocytes. J Immunol 139:4102?4108
  55. Reem GH, Cook LA, Vilcek J (1983) Gamma interferon synthesis by human thymocytes and T lymphocytes inhibited by cyclosporin A. Science 221:63?65
  56. Rimm IJ, Bhan AK, Scheeneberger EE, Schlossman SF, Reinherz EL (1984) Characterization of human thymic epithelial cells grown in serum-free medium. Clin Immunol Immunopathol 31:56?64
  57. Ritter MA, Sauvage CA, Cotmore SF (1981) The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations of epithelial cells. Immunology 44:439?446
  58. Rouse RV, Ewijk W van, Jones PP, Weissman IL (1979) Expression of MHC antigens by mouse thymic dendritic cells. J Immunol 122:2508?2515
  59. Scheiff JM (1976) Multinucleate cells in the thymus of C3H mice. Cell Tissue Res 170:305?314
  60. Scheiff JM (1984) L'épithélium thymique au cours de la croissance et de l'involuation physiologiques et hormono-dépendantes. Thèse, Université de Louvain
  61. Scollay R, Butcher EC, Weissman IL (1980) Thymus cell migration. Quantitative aspects of cellular traffic from the thymus to the periphery in mice. Eur J Immunol 10:210?218
  62. Singer KH, Harden EA, Robertson AL, Lobach DF, Haynes BF (1985) In vitro growth and phenotypic characterization of mesodermal-derived and epithelial components of normal and abnormal human thymus. Hum Immunol 13:161?176
  63. Small M, Barr-Nea L, Aronson M (1984) Culture of thymic epithelial cells from mice and age-related studies on the growing cells. Eur J Immunol 14:936?942
  64. Springer T (1980) Cell surface differentiation in the mouse. In: Kennett RH, McKearn T, Bechtol KS (eds) Monoclonal antibodies. Plenum Press, New York, pp 185?217
  65. Sternberger LA (1979) Immunocytochemistry, 2nd edition, Wiley, New York
  66. Sugimoto M, Nakanishi Y, Otokawa M, Uchida N, Yasuda T, Sato H, Sato Y (1983) Effect of Bordetella pertussis leukocytosis (lymphocytosis)-promoting factor (LPF) on the physical lymphoepithelial-cell association studied with the use of an in vitro model of mouse thymus. J Immunol 130:2767?2774
  67. Sun L, Serrero G, Piltch A, Hayashi J (1987) EGF receptors on TEA3A1 endocrine thymic epithelial cells. Biochem Biophys Res Commun 148:603?608
  68. Sun TT, Bonitz P, Burns WH (1984) Cell culture of mammalian thymic epithelial cells: growth, structural, and antigenic properties. Cell Immunol 83:1?13
  69. Toussaint-Demylle D, Scheiff JM, Haumont S (1990) Thymic nurse cells: morphological study during their isolation from murine thymus. Cell Tissue Res 261:115?123
  70. Toussaint-Demylle D, Scheiff JM, Haumont S (1991) Thymic accessory cell complexes in vitro and in vivo: morphological study. Cell Tissue Res 263:293?301
  71. Ushiki T (1986) A scanning electron-microscopic study of the rat thymus with special reference to cell types and migration of lymphocytes into the general circulation. Cell Tissue Res 244:285?298
  72. Vakharia DD (1983) Demonstration of keratin filaments in thymic nurse cells (TNC) and alloreactivity of TNC-T cell population. Thymus 5:43?52
  73. Vakharia DD, Mitchison NA (1984) Thymic epithelial cells derived from the cultures of thymic nurse cells. Immunol Lett 7:261?266
  74. Vliet E van, Melis M, Ewijk W van (1984a) Immunohistology of thymic nurse cells. Cell Immunol 87:101?109
  75. Vliet E van, Melis M, Ewijk W van (1984b) Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol 14:524?529
  76. Waal Malefijt R de, Leene W, Roholl PJM, Wormmeester J, Hoeben KA (1986) T cell differentiation within thymic nurse cells. Lab Invest 55:25?34
  77. Wasilenko WJ, Marchok AC (1986) Utilization of pyruvate and pyruvate precursors by normal and carcinogen-altered rat tracheal epithelial cells in culture. J Cell Physiol 126:69?76
  78. Wekerle H, Ketelsen UP (1980) Thymic nurse cells ? Ia-bearing epithelium involved in T-lymphocyte differentiation? Nature 283:402?404
  79. Wekerle H, Ketelsen UP, Ernst M (1980) Thymic nurse cells. Lymphoepithelial cell complexes in murine thymuses: morphological and serological charaterization. J Exp Med 151:925?944