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Mutations in chicory FEH genes are statistically associated with enhanced resistance to post-harvest inulin depolymerization

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Dauchot, Nicolas [FUNDP] Raulier, Pierre [UCL] maudoux, Olivier [Chicoline-Cosucra] Notté, Christine [Chicoline-Cosucra] Bertin, Pierre [UCL] Draye, Xavier [UCL] Van Cutsem, Pierre [FUNDP]

Industrial chicory (Cichorium intybus L.) root is the main commercial source of inulin, a linear fructose polymer used as dietary fiber. Post-harvest, inulin is depolymerized into fructose which drastically increases processing cost. To identify genetic variations associated with enhanced susceptibility to post-harvest inulin depolymerization and related free sugars content increase, we used a candidate-gene approach focused on inulin and sucrose synthesis and degradation genes, all members of the family 32 of glycoside hydrolases (GH32). Polymorphism in these genes was first investigated by carrying out EcoTILLING on two groups of chicory breeding lines exhibiting contrasted response to post-harvest inulin depolymerization. This allowed the identification of polymorphisms significantly associated with depolymerization in three fructan exohydrolase genes (FEH). This association was confirmed on a wider panel of 116 unrelated families in which the FEH polymorphism explained 35 % of the postharvest variance for inulin content, 36 % of variance for sucrose content, 18 % for inulin degree of polymerization, 23 % for free fructose content and 22 % for free glucose content. These polymorphisms were associated with significant post-harvest changes of inulin content, inulin chainlength and free sugars content.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès interdit
Publication date 2014
Language Anglais
Journal information "Theoretical and Applied Genetics : international journal of plant breeding research" - Vol. 127, no. 1, p. 125-135 (2014)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 0040-5752
e-issn 1432-2242
Publication status Publié
Affiliation UCL - SST/ELI/ELIA - Agronomy
Links
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Bibliographic reference Dauchot, Nicolas ; Raulier, Pierre ; maudoux, Olivier ; Notté, Christine ; Bertin, Pierre ; et. al. Mutations in chicory FEH genes are statistically associated with enhanced resistance to post-harvest inulin depolymerization. In: Theoretical and Applied Genetics : international journal of plant breeding research, Vol. 127, no. 1, p. 125-135 (2014)
Permanent URL http://hdl.handle.net/2078.1/134502