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Decrease in Akt/PKB signalling in human skeletal muscle by resistance exercise

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Deldicque, Louise [UCL] Atherton, Philip Patel, Rekha Theisen, Daniel [UCL] Nielens, Henri [UCL] Francaux, Marc [UCL]

We analysed the effects of resistance exercise upon the phosphorylation state of proteins associated with adaptive processes from the Akt/PKB (protein kinase B) and the mitogen-activated protein kinase (MAPK) pathways. Nine healthy young men (21.7 +/- 0.55 year) performed 10 sets of 10 leg extensions at 80% of their 1-RM (repetition maximum). Muscle biopsies were taken from the vastus lateralis at rest, within the first 30 s after exercise and at 24 h post-exercise. Immediately post exercise, the phosphorylation states of Akt/PKB on Thr308 and Ser473 and 4E-BP1 on Thr37/46 (eukaryotic initiation factor 4E-binding protein 1) were decreased (-60 to -90%, P < 0.05). Conversely, the phosphorylation of p70(s6k) (p70 ribosomal S6 kinase) on Thr421/Ser424 was increased more than 20-fold (P < 0.05), and this was associated with a 10- to 50-fold increase in the phosphorylation of p38 and ERK1/2 (extracellular signal-regulated kinase) (P < 0.05). Twenty-four hours post-exercise the phosphorylation state of Akt/PKB on Thr308 was depressed, whereas the phosphorylation of p70(s6k) on Thr421/Ser424 and sarcoplasmic ERK1/2 were elevated. The present results indicate that high-intensity resistance exercise in the fasted state inhibits Akt/PKB and 4E-BP1 whilst concomitantly augmenting MAPK signalling and p70(s6k) on Thr421/Ser424.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2008
Language Anglais
Journal information "European Journal of Applied Physiology" - Vol. 104, no. 1, p. 57-65 (2008)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 1439-6319
e-issn 1439-6327
Publication status Publié
Affiliations UCL - (SLuc) Service de médecine physique et de réadaptation motrice
UCL - MD/IEPR - Institut d'éducation physique et de réadaptation
UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur
MESH Subject p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors - metabolism ; Signal Transduction - drug effects ; Ribosomal Protein S6 Kinases, 70-kDa - metabolism ; Quadriceps Muscle - enzymology - metabolism ; Proto-Oncogene Proteins c-akt - metabolism ; Protein Kinase Inhibitors - pharmacology ; Phosphorylation ; Phosphoproteins - metabolism ; Myoblasts, Skeletal - drug effects - metabolism ; Muscle Contraction ; Mitogen-Activated Protein Kinase 3 - antagonists & inhibitors - metabolism ; Mitogen-Activated Protein Kinase 1 - antagonists & inhibitors - metabolism ; Mice ; Male ; Humans ; Fasting - metabolism ; Exercise - physiology ; Cell Line ; Animals ; Adult ; Adaptor Proteins, Signal Transducing - metabolism
Links
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Bibliographic reference Deldicque, Louise ; Atherton, Philip ; Patel, Rekha ; Theisen, Daniel ; Nielens, Henri ; et. al. Decrease in Akt/PKB signalling in human skeletal muscle by resistance exercise. In: European Journal of Applied Physiology, Vol. 104, no. 1, p. 57-65 (2008)
Permanent URL http://hdl.handle.net/2078.1/12638