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LAGE-1, a new gene with tumor specificity.

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Lethé, B Lucas, Sophie [UCL] Michaux, Lucienne [UCL] De Smet, Charles [UCL] Godelaine, D. De Plaen, Etienne [UCL] Boon, Thierry [UCL]

Representational difference analysis was used to identify genes that are expressed in a human melanoma cell line and not in normal skin. A cDNA clone that appeared to be specific for tumors was obtained and the corresponding gene was sequenced. This new gene was named LAGE-I. Using a LAGE-I probe to screen a cDNA library from the same melanoma cell line, we identified a closely related gene, which proved to be identical to NY-ESO-I, a gene recently reported to code for an antigen recognized by autologous antibodies in an esophageal squamous cell carcinoma. Gene LAGE-I maps to Xq28. It comprises 3 exons. Alternative splicing produces 2 major transcripts encoding polypeptides of 210 and 180 residues, respectively. Expression of LAGE-I was observed in 25-50% of tumor samples of melanomas, non-small-cell lung carcinomas, bladder, prostate and head and neck cancers. The only normal tissue that expressed the gene was testis. As for MAGE-AI, expression of LAGE-I is induced by deoxy-azacytidine in lymphoblastoid cells, suggesting that tumoral expression is due to demethylation. The expression of LAGE-I is strongly correlated with that of NY-ESO-I. It is also clearly correlated with the expression of MAGE genes.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1998
Journal information "International journal of cancer. Journal international du cancer" - Vol. 76, no. 6, p. 903-8 (1998)
Peer reviewed yes
issn 0020-7136
Publication status Publié
Affiliations UCL - MD/MINT - Département de médecine interne
UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Amino Acid Sequence ; Antigens, Neoplasm ; Antigens, Surface ; Base Sequence ; Chromosome Mapping ; Humans ; Melanoma - genetics ; Membrane Proteins ; Molecular Sequence Data ; Neoplasms - genetics ; Oncogenes ; Proteins - genetics ; Tumor Cells, Cultured
Links
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Bibliographic reference Lethé, B ; Lucas, Sophie ; Michaux, Lucienne ; De Smet, Charles ; Godelaine, D. ; et. al. LAGE-1, a new gene with tumor specificity.. In: International journal of cancer. Journal international du cancer, Vol. 76, no. 6, p. 903-8 (1998)
Permanent URL http://hdl.handle.net/2078.1/12439