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A new class of proteins regulating gene expression in enterobacteria

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Mikulskis, Alvidas V. Cornelis, Guy [UCL]

YmoA and Hha are highly similar bacterial proteins downregulating gene expression in Yersinia enterocolitica and Escherichia coli, respectively. The phenotype of ymoA mutants evokes that of mutants affected in some histone-like proteins. This paper describes complementation of a ymoA mutation in Y. enterocolitica by the hha gene from E. coli. We show that YmoA and Hha are not only very similar proteins but that they are functionally interchangeable. Genetic experiments indicate that Hha can also stimulate transposition events in vivo. By Southern blot analysis we detected hha-homologous genes at least in Citrobacter diversus, Shigella flexneri, Shigella dysenteriae, Klebsiella pneumoniae and Salmonella typhimurium. We suggest that both YmoA and Hha belong to a new family of proteins downregulating gene expression in different enterobacteria.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1994
Journal information "Molecular Microbiology" - Vol. 11, no. 1, p. 77-86 (1994)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing Ltd. (Chichester)
issn 0950-382X
e-issn 1365-2958
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Amino Acid Sequence ; Bacterial Proteins - genetics ; Base Sequence ; Chromosomes, Bacterial ; Cloning, Molecular ; Conjugation, Genetic ; DNA Transposable Elements - genetics ; DNA, Bacterial - isolation & purification ; DNA-Binding Proteins ; Enterobacteriaceae - genetics ; Escherichia coli - genetics ; Escherichia coli Proteins ; Gene Expression Regulation, Bacterial - genetics ; Genes, Bacterial - genetics ; Genetic Complementation Test ; Molecular Sequence Data ; Mutation ; Recombinant Fusion Proteins - genetics ; Transcription, Genetic - genetics ; Yersinia enterocolitica - genetics
Links
  1. Blanco A, Overproduction of a Clostridium cellulolyticum endoglucanase by mutant strains of Escherichia coli, 10.1016/0378-1097(91)90306-u
  2. Carmona M., Mol Microbiol, 9, 1011 (1993)
  3. China B., Mol Microbiol, 4, 1585 (1990)
  4. China B., Infect Immun, 61, 3129 (1993)
  5. Cornelis G., Colson C., Restriction of DNA in Yersinia enterocolitica Detected Recipient Ability for a Derepressed R Factor from Escherichia coli, 10.1099/00221287-87-2-285
  6. Cornelis Guy, Sory M.P., Laroche Y., Derclaye I., Genetic analysis of the plasmid region controlling virulence in Yersinia enterocolitica 0:9 by Mini-Mu insertions and lac gene fusions, 10.1016/0882-4010(86)90067-7
  7. Cornelis Guy, Vanootegem J.-C., Sluiters C., Transcription of the yop regulon from Y. enterocolitica requires trans acting pYV and chromosomal genes, 10.1016/0882-4010(87)90078-7
  8. Cornelis G.R., Mol Microbiol, 3, 1455 (1989)
  9. Cornelis G.R., Mol Microbiol, 5, 1023 (1991)
  10. F. la Cruz, M. Carmona, and A. Juarez (1992 ) The Hha protein fromEscherichia coliis highly homologous to the YmoA protein from Yersinia enterocotitica .Mol Micro Biol6: 3451 -3452 .
  11. Delor I., Infect Immun, 58, 2983 (1990)
  12. Dersch P., Mol Microbiol, 8, 875 (1993)
  13. Dorman Charles J., Bhriain Niamh Ní, DNA topology and bacterial virulence gene regulation, 10.1016/0966-842x(93)90114-7
  14. Dorman Charles J., Bhriain Niamh Ni, Higgins Christopher F., DNA supercoiling and environmental regulation of virulence gene expression in Shigella flexneri, 10.1038/344789a0
  15. Galas J.D., Mobile DNA, 109 (1989)
  16. Gay P., J Bacteriol, 164, 918 (1985)
  17. Goguen J.D., J Bacteriol, 160, 842 (1984)
  18. Göransson Mikael, Sondén Berit, Nilsson Peter, Dagberg Björn, Foreman Kristina, Emanuelsson Karin, Uhlin Bernt Eric, Transcriptional silencing and thermoregulation of gene expression in Escherichia coli , 10.1038/344682a0
  19. Higgins Christopher F., Dorman Charles J., Stirling Douglas A., Waddell Lesley, Booth Ian R., May Gerhard, Bremer Erhard, A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli, 10.1016/0092-8674(88)90470-9
  20. Higgins C.F., Mol Micro Biol, 4, 2007 (1990)
  21. Hulton Christopher S.J., Seirafi Alexander, Hinton Jay C.D., Sidebotham Julie M., Waddell Lesley, Pavitt Graham D., Owen-Hughes Thomas, Spassky Annick, Buc Henri, Higgins Christopher F., Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteria, 10.1016/0092-8674(90)90458-q
  22. Maureili AT., Proc Natt Acad Sci USA, 85, 2820 (1988)
  23. Michiels T., J Bacteriol, 173, 1677 (1991)
  24. Michiels T., Infect Immun, 58, 2840 (1990)
  25. J.H. Miller (1972 ) InExperiments in Molecular Genetics . Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp.352 -355 .
  26. Nieto J, Secretion of an Aeromonas hydrophila aerolysin by a mutant strain of Escherichia coli, 10.1016/0378-1097(87)90085-1
  27. Nieto J.M., Mol Micro Biol, 5, 1285 (1991)
  28. Pettijohn D.E., J Biol Chem, 263, 12793 (1988)
  29. Prentki Pierre, Teter Bruce, Chandler Michael, Galas David J., Functional promoters created by the insertion of transposable element IS1, 10.1016/0022-2836(86)90134-8
  30. Simon R., Priefer U., Pühler A., A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria, 10.1038/nbt1183-784
  31. Vanooteghem J.-C, J Bacteriol, 172, 3600 (1990)
  32. Yother J., J Bacteriol, 165, 443 (1986)
Bibliographic reference Mikulskis, Alvidas V. ; Cornelis, Guy. A new class of proteins regulating gene expression in enterobacteria. In: Molecular Microbiology, Vol. 11, no. 1, p. 77-86 (1994)
Permanent URL http://hdl.handle.net/2078.1/12019