User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach

Primary tabs

download
  • Open access
  • PDF
  • 6.14 M
Gilon, Patrick [UCL] Mallefet, Jérôme [UCL] De Vriendt, C. [UCL] Pauwels, S. [UCL] Geffard, M Remacle, Claude [UCL]

There are now increasing evidences suggesting that GABA is able of direct interaction with certain endocrine cells. In the present study, highly specific anti-GABA-glutaraldehyde antibodies and 3H-GABA uptake were used at the light and electron microscope levels to investigate the occurrence of cells containing endogenous GABA or taking up exogenous GABA in the mucosal antrum and corpus of the rat stomach. Only certain endocrine cell types of both regions were immunostained or grain-labelled. However, the morphology of their secretory granules did not allow to identify the nature of their hormone with certainty but suggested that somatostatin-like cells could interact with GABA. The combination of gastrin and somatostatin immunodetection with 3H-GABA uptake autoradiography at the light microscope level, revealed that a subpopulation of somatostatin-like cells and other still unidentified endocrine cells are able to take up GABA, while the gastrin-like cells are not. These results reinforce the hypothesis that certain endocrine cell types of the diffuse endocrine system of the digestive tract are able to directly interact with GABA.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 1990
Language Anglais
Journal information "Histochemistry" - Vol. 93, no. 6, p. 645-654 (1990)
Peer reviewed yes
Publisher Springer ((Germany) Berlin)
issn 0301-5564
Publication status Publié
Affiliations UCL - MD/FSIO - Département de physiologie et pharmacologie
UCL - SC/BIOL - Département de biologie
MESH Subject APUD Cells - metabolism - ultrastructure ; Animals ; Autoradiography ; Gastric Mucosa - metabolism - ultrastructure ; Gastrins - analysis ; Immunohistochemistry ; Microscopy, Electron ; Rats ; Rats, Inbred Strains ; Somatostatin - analysis ; gamma-Aminobutyric Acid - metabolism
Links
  1. Anderson RA, Mitchell R (1986) Distribution of GABA binding site subtypes in rat pituitary gland. Brain Res 365:78?84
  2. Davanger S, Ottersen OP, Storm-Mathisen J (1987) Immunocyto-chemical localization of GABA in cat myenteric plexus. Neurosci Lett 73:27?32
  3. Davanger S, Ottersen OP, Storm-Mathisen J (1989) GABA-immunoreactive cells in the rat gastrointestinal epithelium. Anat Embryol 179:221?226
  4. Eldred WD, Zucker C, Karten HJ, Yazulla S (1983) Comparison of fixation and penetration enhancement techniques for use in ultrastructural immunocytochemistry. J Histochem Cytochem 31:285?292
  5. Erdö SL (1985) Peripheral GABAergic mechanisms. Trends Pharmacol Sci 6:205?208
  6. Erdö SL, Woff JR (1988) Releasable, non-neuronal GABA pool in rat stomach. Eur J Pharmacol 156:165?168
  7. Erdö SL, Ezer E, Matuz J, Wolff JR, Amenta F (1989) GABAA receptors in the rat stomach may mediate mucoprotective effects. Eur J Pharmacol 165:79?86
  8. Gamrani H, Harandi M, Belin MF, Dubois MP, Calas A (1984) Direct electron microscopic evidence for the coexistence of GABA uptake and endogenous serotonin in the same rat central neurons by coupled radioautographic and immunocytochemical procedures. Neurosci Lett 48:25?30
  9. Gamrani H, Onteniente B, Seguela P, Geffard M, Calas A (1986) Gamma-aminobutyric acid-immunoreactivity in the rat hippocampus. A light and electron microscopic study with anti-GABA antibodies. Brain Res 364:30?38
  10. Garry DJ, Sorenson RL, Coulter HD (1987a) Ultrastructural localization of gamma amino butyric acid immunoreactivity in B cells of the rat pancreas. Diabetologia 30:115?119
  11. Garry DJ, Coulter HD, McIntee TJ, Wu J-Y, Sorenson RL (1987b) Immunoreactive GABA transaminase within the pancreatic islet is localized in mitochondria of the B-cell. J Histochem Cytochem 35:831?836
  12. Gilon P, Remacle C, Janssens de Varebeke P, Pauwels G, Hoet JJ (1987a) GABA content and localisation of high-affinity GABA uptake during the development of the rat pancreas. Cell Mol Biol 33:573?585
  13. Gilon P, Reusens-Billen B, Remacle C, Janssens de Varebeke P, Pauwels G, Hoet JJ (1987b) Localization of high-affinity GABA uptake and GABA content in the rat duodenum during development. Cell Tissue Res 249:593?600
  14. Gilon P, Campistron G, Geffard M, Remacle C (1988) Immunocytochemical localisation of GABA in endocrine cells of the rat entero-pancreatic system. Biol Cell 62:265?273
  15. Gilon P, Remacle C (1989) High-affinity GABA uptake in a subpopulation of somatostatin cells in rat pancreas. J Histochem Cytochem 37:1133?1139
  16. Grube D, Forssmann WG (1979) Morphology and function of the entero-endocrine cells. Horm Metab Res 11:589?606
  17. Guo Y-S, Thompson JC, Singh P (1989) Effect of ?-aminobutyric acid on bombesin-evoked release of somatostatin and gastrin from isolated rat stomach. Regul Pept 24:179?186
  18. Harty RF, Franklin PA (1983) GABA affects the release of gastrin and somatostatin from rat antral mucosa. Nature 303:623?624
  19. Harty RF, Franklin PA (1986) Cholinergic mediation of ?-aminobutyric acid-induced gastrin and somatostatin release from rat antrum. Gastroenterology 91:1221?1226
  20. Harty RF, Murthy SNS (1986) GABAergic mechanisms in the endocrine stomach and duodenum: their possible functional significance. In: Erdö SL, Bowery NG (eds) GABAergic mechanisms in the mammalian periphery. Raven Press, New York, pp 339?352
  21. Hills JM, Jessen KR, Mirsky R (1987) An immunohistochemical study of the distribution of enteric GABA-containing neurons in the rat and guinea-pig intestine. Neuroscience 22:301?312
  22. Jessen KR, Mirsky R, Hills JM (1987) GABA as an autonomic neurotransmitter: studies on intrinsic GABAergic neurons in the myenteric plexus of the gut. Trends Neurosci 10:255?262
  23. Jessen KR, Hills JM, Limbrick AR (1988) GABA immunoreactivity and 3H-GABA uptake in mucosal epithelial cells of the rat stomach. Gut 29:1549?1556
  24. Kakucska I, Tappaz ML, Gaal G, Stoeckel ME, Makara GB (1988) GABAergic innervation of somatostatin-containing neurosecretory cells of the anterior periventricular hypothalamic area: a light an electron microscopy double immunolabelling study. Neuroscience 25:585?593
  25. Kisvarday ZF, Cowey A, Hodgson AJ, Somogyi P (1986) The relationship between GABA immunoreactivity and labelling by local uptake of (3H)GABA in the striate cortex of monkey. Exp Brain Res 62:89?98
  26. Kusunoki M, Yamamura T, Ichii S, Fujita S, Nakai T, Utsunomiya J (1988) The effects of sodium valproate on plasma somatostatin and insulin in humans. J Clin Endocrinol Metab 67:1060?1063
  27. Larsson L-I (1977) Ontogeny of peptide-producing nerves and endocrine cells of the gastro-duodeno-pancreatic region. Histochemistry 54:133?142
  28. Le Douarin NM (1988) On the origin of pancreatic endocrine cells. Cell 53:169?171
  29. Lloyd KG, Prouteau M, Delahaye M, Magnier G, Voltz C (1986) GABA, gastric ulceration, and gastric acid secretion. In: Erdö SL, Bowery NG (eds) GABAergic mechanisms in the mammalian periphery. Raven Press, New York, pp 353?364
  30. McCann SM, Rettori V (1986) Gamma amino butyric acid (GABA) controls anterior pituitary hormone secretion. In: Racagni G, Donoso AO (eds) GABA and endocrine function. Raven Press, New York, pp 173?189
  31. Melis GB, Mais V, Paoletti AM, Antinori D, de Ruggiero A, Fioretti P (1986) GABAergic control of anterior pituitary function in humans. In: Racagni G, Donoso AO (eds) GABA and endocrine function. Raven Press, New York, pp 219?242
  32. Minano FJ, Serrano JS, Pascual J, Sancibrian M (1987) Effects of GABA on gastric acid secretion and ulcer formation in rats. Life Sci 41:1651?1658
  33. Nanopoulos D, Belin MF, Maitre M, Vincendon G, Pujol JF (1982) Immunocytochemical evidence for the existence of GABA-ergic neurons in the nucleus raphe dorsalis. Possible existence of neurons containing serotonin and GABA. Brain Res 232:375?389
  34. Navone F, Jahn R, Di Gioia G, Stukenbrok H, Greengard P, De Camilli P (1986) Protein p38: an integral membrane protein specific for small vesicles of neurons and neuroendocrine cells. J Cell Biol 103:2511?2527
  35. Okada Y (1986) Localization and function of GABA in the pancreatic islets. In: Erdö SL, Bowery NG (eds) GABAergic mechanisms in the mammalian periphery, Raven Press, New York, pp 223?240
  36. Rahier J, Pauwels S, Dockray GJ (1987) Biosynthesis of gastrin. Localization of the precursor and peptide products using electron microscopic-immunogold methods. Gastroenterology 92:1146?1152
  37. Rubin W, Schwartz B (1983) Identification of the serotonin-synthesizing endocrine cells in the rat stomach by electron microscopic radioautography and amine fluorescence. Gastroenterology 84:34?50
  38. Rubin W, Schwartz B (1984) Identification of the APUD endocrine cells of rat fundic mucosa by means of combined amine fluorescence and electron microscopy. J Histochem Cytochem 32:67?75
  39. Seguela P, Geffard M, Buijs RM, Le Moal M (1984) Antibodies against ?-aminobutyric acid: specificity studies and immunocy-tochemical results. Proc Natl Acad Sci USA 81:3888?3892
  40. Solcia E, Capella C, Vassallo G, Buffa R (1975) Endocrine cells of the gastric mucosa. Int Rev Cytol 42:223?286
  41. Solcia E, Capella C, Buffa R, Usellini L, Fiocca R, Sessa F (1981) Endocrine cells of the digestive system. In: Johnson LR (ed) Physiology of the gastrointestinal tract. Raven Press, New York, pp 39?58
  42. Solcia E, Usellini L, Buffa R, Rindi G, Villani L, Zampatti C, Silini E (1987) Endocrine cells producing regulatory peptides. Experientia 43:839?850
  43. Taniguchi H, Yoshioka M, Ejiri K, Ishihara K, Tamagawa M, Hirose Y, Ishihara K, Utsumi M, Baba S, Okada Y (1982) Suppression of somatostatin release and increase of somatostatin content in pancreatic islets by GABA. In: Okada Y, Roberts E (eds) Problems in GABA research from brain to bacteria. Excerpta Medica, ICS 565, Amsterdam, pp 406?412
  44. Teitelman G, Joh TH, Reis DJ (1981) Transformation of catecholaminergic precursors into glucagon (A) cells in mouse embryonic pancreas. Proc Natl Acad Sci USA 78:5225?5229
  45. Thirlby RC, Stevens MH, Blair AJ, Petty F, Crawford IL, Taylor IL, Walsh JH, Feldman M (1988) Effect of GABA on basal and vagally mediated gastric acid secretion and hormone release in dogs. Am J Physiol 254:G723?731
  46. Vincent SR, Hökfelt T, Wu J-Y, Elde RP, Morgan LM, Kimmel JR (1983) Immunocytochemical studies of the GABA system in the rat pancreas. Neuroendocrinology 36:197?204
  47. Wu J-Y, Lin C-T, Lin H, Xu Y, Liu J-W, Hwang BH, Wei SC (1986) Immunochemical characterization and immunohistochemical localization of glutamate decarboxylase and GABA transaminase in peripheral tissues. In: Erdö SL, Bowery NG (eds) GABAergic mechanisms in the mammalian periphery, Raven Press, New York, pp 19?34
Bibliographic reference Gilon, Patrick ; Mallefet, Jérôme ; De Vriendt, C. ; Pauwels, S. ; Geffard, M ; et. al. Immunocytochemical and autoradiographic studies of the endocrine cells interacting with GABA in the rat stomach. In: Histochemistry, Vol. 93, no. 6, p. 645-654 (1990)
Permanent URL http://hdl.handle.net/2078.1/11426