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Antagonistic effects of leucine and glutamine on the mTOR pathway in myogenic C(2)C (12) cells.

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Deldicque, Louise [UCL] Sanchez Canedo, Cossette [UCL] Horman, Sandrine [UCL] De Potter, Isabelle [UCL] Bertrand, Luc [UCL] Hue, Louis [UCL] Francaux, Marc [UCL]

This study compared the effects of leucine and glutamine on the mTOR pathway, on protein synthesis and on muscle-specific gene expression in myogenic C(2)C(12) cells. Leucine increased the phosphorylation state of mTOR, on both Ser2448 and Ser2481, and its downstream effectors, p70(S6k), S6 and 4E-BP1. By contrast, glutamine decreased the phosphorylation state of mTOR on Ser2448, p70(S6k) and 4E-BP1, but did not modify the phosphorylation state of mTOR on Ser2481 and S6. Whilst the phosphorylation state of the mTOR pathway is usually related to protein synthesis, the incorporation of labelled methionine/cysteine was only transiently modified by leucine and was unaltered by glutamine. However, these two amino acids affected the mRNA levels of desmin, myogenin and myosin heavy chain in a time-dependant manner. In conclusion, leucine and glutamine have opposite effects on the mTOR pathway. Moreover, they induce modification of muscle-specific gene expression, unrelated to their effects on the mTOR/p70(S6k) pathway.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2008
Language Anglais
Journal information "Amino Acids" - Vol. 35, no. 1, p. 147-155 (2008)
Peer reviewed yes
Publisher Springer Wien ((Austria) Vienna)
issn 0939-4451
e-issn 1438-2199
Publication status Publié
Affiliations UCL - MD/IEPR - Institut d'éducation physique et de réadaptation
UCL - MD/BICL - Département de biochimie et de biologie cellulaire
UCL - MD/MINT - Département de médecine interne
MESH Subject Protein Kinases - metabolism ; Phosphorylation - drug effects ; Myoblasts, Skeletal ; Muscle Proteins - biosynthesis ; Mice ; Leucine - antagonists & inhibitors - pharmacology ; Glutamine - antagonists & inhibitors - pharmacology ; Gene Expression Regulation - drug effects ; Drug Antagonism ; Cell Line ; Animals
Keywords 4E-BP1 ; Amino acids ; Gene expression ; p70S6k ; Protein synthesis
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Bibliographic reference Deldicque, Louise ; Sanchez Canedo, Cossette ; Horman, Sandrine ; De Potter, Isabelle ; Bertrand, Luc ; et. al. Antagonistic effects of leucine and glutamine on the mTOR pathway in myogenic C(2)C (12) cells.. In: Amino Acids, Vol. 35, no. 1, p. 147-155 (2008)
Permanent URL http://hdl.handle.net/2078.1/11177