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Coagulation function in fresh-frozen plasma prepared with two photochemical treatment methods: methylene blue and amotosalen.

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Osselaer, Jean-Claude Debry, Cécile Goffaux, Maite Pineau, Jacqueline Calomme, Genevieve Chatelain, Bernard [UCL] Lin, Lily

BACKGROUND: Pathogen inactivation of plasma intended for transfusion is now the standard of care in Belgium. Two methods for treatment of single plasma units are available: amotosalen plus ultraviolet A light and methylene blue plus visible light. This study compared the quality and stability of plasma treated with these two methods. STUDY DESIGN AND METHODS: Plasma units made from a pool of two ABO-matched fresh apheresis units were photochemically treated with either amotosalen (PCT-FFP) or methylene blue (MB-FFP). A total of 12 paired samples were evaluated. Plasma coagulation function was assessed at three time points: immediately after treatment, after 30 days of frozen storage, and an additional 24 hours at 4 degrees C after thawing. Comparison between PCT-FFP and MB-FFP was assessed with the paired t test and a p value of less than 0.05 indicated statistical significance. RESULTS: Based on statistical analysis, mean levels of factor (F)II, FXII, FXIII, von Willebrand antigen, ADAMTS-13, D-dimers, and protein C were equivalent between PCT-FFP and MB-FFP for all three time points. PCT-FFP exhibited shorter mean prothrombin time, activated partial thromboplastin time (two time points), and thrombin time and higher mean levels of fibrinogen, FXI, and protein S than MB-FFP. Retention of FV, FVII, FVIII, FX, or von Willebrand factor:ristocetin cofactor in PCT-FFP was either equivalent to or higher than MB-FFP. MB-FFP contained higher mean levels of plasminogen, antithrombin, and plasmin inhibitor than PCT-FFP. Retention of F IX in MB-FFP was higher than PCT-FFP only after the 4 degrees C storage after thawing. CONCLUSION: There is adequate preservation of therapeutic coagulation factor activities in both PCT-FFP and MB-FFP. The overall coagulation factor levels and stability of PCT-FFP were better preserved than MB-FFP.

Document type Article de périodique (Journal article) – Comparative Study, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 2008
Journal information "Transfusion" - Vol. 48, no. 1, p. 108-17 (2008)
Peer reviewed yes
issn 0041-1132
Publication status Publié
Affiliations UCL - MD/BICL - Département de biochimie et de biologie cellulaire
UCL - (MGD) Centre de transfusion sanguine
UCL - (MGD) Laboratoire de biologie clinique
MESH Subject Blood Coagulation ; Blood Coagulation Factors - analysis ; Blood Coagulation Tests ; Humans ; Light ; Methylene Blue ; Photochemistry - methods ; Plasma - physiology - virology ; Psoralens ; Ultraviolet Rays ; Virus Inactivation
Links
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Bibliographic reference Osselaer, Jean-Claude ; Debry, Cécile ; Goffaux, Maite ; Pineau, Jacqueline ; Calomme, Genevieve ; et. al. Coagulation function in fresh-frozen plasma prepared with two photochemical treatment methods: methylene blue and amotosalen.. In: Transfusion, Vol. 48, no. 1, p. 108-17 (2008)
Permanent URL http://hdl.handle.net/2078.1/11129