User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Fe65 does not stabilize AICD during activation of transcription in a luciferase assay.

Primary tabs

Huysseune, Sandra [UCL] Kienlen-Campard, Pascal [UCL] Octave, Jean-Noël [UCL]

The APP intracellular domain (AICD) could be involved in signaling via interaction with the adaptor protein Fe65, and with the histone acetyl transferase Tip60. However, the real function of AICD and Fe65 in regulation of transcription remains controversial. In this study, the human APPGal4 fusion protein was expressed in CHO cells and the transcriptional activity of AICDGal4 was measured in a luciferase-based reporter assay. AICDGal4 was stabilized by expression of Fe65 and levels of AICDGal4 controlled luciferase activity. On the contrary, when human APP was expressed in CHO cells, coexpression of Fe65 increased luciferase activity without affecting the amount of AICD fragment. AICD produced from APP was protected from degradation by orthophenanthroline, but not by lactacystine, indicating that AICD is not a substrate of the chymotryptic activity of the proteasome. It is concluded that Fe65 can control luciferase activity without stabilizing the labile AICD fragment.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2007
Journal information "Biochemical and biophysical research communications" - Vol. 361, no. 2, p. 317-22 (2007)
Peer reviewed yes
issn 0006-291X
Publication status Publié
Affiliation UCL - MD/FSIO - Département de physiologie et pharmacologie
MESH Subject Amyloid beta-Protein Precursor - chemistry - genetics ; Animals ; Biological Assay ; CHO Cells ; Cricetinae ; Cricetulus ; Humans ; Luciferases - genetics - metabolism ; Nuclear Proteins - metabolism ; Peptide Fragments - chemistry - genetics ; Phenanthrolines - pharmacology ; Proteasome Endopeptidase Complex - metabolism ; Protein Structure, Tertiary ; Recombinant Fusion Proteins - metabolism ; Substrate Specificity - drug effects ; Thermodynamics ; Trans-Activation (Genetics) - drug effects - genetics
Links
Bibliographic reference Huysseune, Sandra ; Kienlen-Campard, Pascal ; Octave, Jean-Noël. Fe65 does not stabilize AICD during activation of transcription in a luciferase assay.. In: Biochemical and biophysical research communications, Vol. 361, no. 2, p. 317-22 (2007)
Permanent URL http://hdl.handle.net/2078.1/11038