Helodermin-loaded nanoparticles: characterization and transport across an in vitro model of the follicle-associated epithelium

des Rieux, Anne [UCL] Fievez, Virginie [UCL] Momtaz, Maryam [UCL] Detrembleur, Christophe Alonso-Sande, Maria Van Gelder, Jan Cauvin, Annick Schneider, Yves-Jacques [UCL] Préat, Véronique [UCL] et al.[show all]

M cells represent a potential portal for oral delivery of peptides and proteins due to their high endocytosis abilities. An in vitro model of human FAE (co-cultures) was used to evaluate the influence of M cells on the transport of free and encapsulated helodermin--a model peptide--across the intestinal epithelium. M cells enhanced transport of intact helodermin (18-fold, Papp=3 x 10(-6) cm s(-1)). As pegylation increased nanoparticle transport by M cells, helodermin was encapsulated in 200 nm nanoparticles containing PEG-b-PLA:PLGA 1:1. Stability of the selected formulation was demonstrated in simulated gastric and intestinal fluids. M cells increased the transport of helodermin encapsulated in these nanoparticles by a factor of 415, as compared to Caco-2 cells. Transport of free and encapsulated helodermin occurred most probably by endocytosis. In conclusion, M cells improved helodermin transport across the intestinal epithelium, confirming their high potential for oral delivery of peptides.