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Rhubarb extract prevents hepatic inflammation induced by acute alcohol intake, an effect related to the modulation of the gut microbiota.

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Neyrinck, Audrey M. [UCL] Etxeberria, Usune [UCL] Taminiau, Bernard Daube, Georges Van Hul, Matthias [UCL] Everard, Amandine [UCL] Cani, Patrice D. [UCL] Bindels, Laure B. [UCL] Delzenne, Nathalie M. [UCL]

(eng) SCOPE: Binge consumption of alcohol is an alarming global health problem. Acute ethanol intoxication is characterized by inflammation and oxidative stress, which could be promoted by gut barrier function alterations. In this study, we have tested the hypothesis of the hepatoprotective effect of rhubarb extract in a mouse model of binge drinking and we explored the contribution of the gut microbiota in the related- metabolic effects. METHODS AND RESULTS: Mice were fed a control diet supplemented with or without 0.3% rhubarb extract for 17 days and were necropsied 6 hours after an alcohol challenge. Supplementation with rhubarb extract changed the microbial ecosystem (assessed by 16S rDNA pyrosequencing) in favor of Akkermansia muciniphila and Parabacteroides goldsteinii. Furthermore, it improved alcohol-induced hepatic injury, down-regulated key markers of both inflammatory and oxidative stresses in the liver tissue, without affecting significantly steatosis. In the gut, rhubarb supplementation increased crypt depth, tissue weight, the expression of antimicrobial peptides. CONCLUSIONS: These findings suggest that some bacterial genders involved in gut barrier function, are promoted by phytochemicals present in rhubarb extract, and could therefore be involved in the modulation of the susceptibility to hepatic diseases linked to acute alcohol consumption. This article is protected by copyright. All rights reserved.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2017
Language Anglais
Journal information "Molecular Nutrition & Food Research" - Vol. 61, no. 1, p. 1500899 [1-12] (2017)
Peer reviewed yes
Publisher Wiley - V C H Verlag GmbH & Co. KGaA ((Germany) Weinheim)
issn 1613-4125
e-issn 1613-4133
Publication status Publié
Affiliation UCL - SSS/LDRI - Louvain Drug Research Institute
Keywords Akkermansia muciniphila ; Alcoholic liver disease ; Antimicrobial peptides ; Gut barrier ; Microbiota ; Parabacteroides goldsteinii ; Steatosis
Links
  1. Shukla Shivendra D., Pruett Stephen B., Szabo Gyongyi, Arteel Gavin E., Binge Ethanol and Liver: New Molecular Developments, 10.1111/acer.12011
  2. Diagnosis and Management of Alcoholic Liver Disease, 10.14218/jcth.2015.00008
  3. Mantena Sudheer K., King Adrienne L., Andringa Kelly K., Eccleston Heather B., Bailey Shannon M., Mitochondrial dysfunction and oxidative stress in the pathogenesis of alcohol- and obesity-induced fatty liver diseases, 10.1016/j.freeradbiomed.2007.12.029
  4. Donohue Terrence M., Osna Natalia A., Trambly Casey S., Whitaker Nash P., Thomes Paul G., Todero Sandra L., Davis John S., Early Growth Response-1 Contributes to Steatosis Development After Acute Ethanol Administration : Egr-1 EXPRESSION AFTER EtOH TREATMENT, 10.1111/j.1530-0277.2011.01681.x
  5. Jr Terrence M Donohue, Alcohol-induced steatosis in liver cells, 10.3748/wjg.v13.i37.4974
  6. Stewart Stephen, Jones David, Day Chris P, Alcoholic liver disease: new insights into mechanisms and preventative strategies, 10.1016/s1471-4914(01)02096-2
  7. Marmier Solenne, Dentin Renaud, Daujat-Chavanieu Martine, Guillou Hervé, Bertrand-Michel Justine, Gerbal-Chaloin Sabine, Girard Jean, Lotersztajn Sophie, Postic Catherine, Novel role for carbohydrate responsive element binding protein in the control of ethanol metabolism and susceptibility to binge drinking : STEATOHEPATITIS/METABOLIC LIVER DISEASE, 10.1002/hep.27778
  8. Vassallo G., Mirijello A., Ferrulli A., Antonelli M., Landolfi R., Gasbarrini A., Addolorato G., Review article: alcohol and gut microbiota - the possible role of gut microbiota modulation in the treatment of alcoholic liver disease, 10.1111/apt.13164
  9. Massey Veronica L., Arteel Gavin E., Acute Alcohol-Induced Liver Injury, 10.3389/fphys.2012.00193
  10. MCC Canesso, NL Lacerda, CM Ferreira, JL Gonçalves, D Almeida, C Gamba, G Cassali, SH Pedroso, C Moreira, FS Martins, JR Nicoli, MM Teixeira, ALB Godard, AT Vieira, Comparing the effects of acute alcohol consumption in germ-free and conventional mice: the role of the gut microbiota, 10.1186/s12866-014-0240-4
  11. Hammer Adam M., Khan Omair M., Morris Niya L., Li Xiaoling, Movtchan Nellie V., Cannon Abigail R., Choudhry Mashkoor A., The Effects of Alcohol Intoxication and Burn Injury on the Expression of Claudins and Mucins in the Small and Large Intestines : , 10.1097/shk.0000000000000483
  12. Leclercq Sophie, Matamoros Sébastien, Cani Patrice D., Neyrinck Audrey M., Jamar François, Stärkel Peter, Windey Karen, Tremaroli Valentina, Bäckhed Fredrik, Verbeke Kristin, de Timary Philippe, Delzenne Nathalie M., Intestinal permeability, gut-bacterial dysbiosis, and behavioral markers of alcohol-dependence severity, 10.1073/pnas.1415174111
  13. Gao Bin, Bataller Ramon, Alcoholic Liver Disease: Pathogenesis and New Therapeutic Targets, 10.1053/j.gastro.2011.09.002
  14. Pan Min-Hsiung, Lai Ching-Shu, Ho Chi-Tang, Anti-inflammatory activity of natural dietary flavonoids, 10.1039/c0fo00103a
  15. YAO L. H., JIANG Y. M., SHI J., TOM�S-BARBER�N F. A., DATTA N., SINGANUSONG R., CHEN S. S., Flavonoids in Food and Their Health Benefits, 10.1007/s11130-004-0049-7
  16. Ding Ren-Bo, Tian Ke, huang Li-Li, He Cheng-Wei, Jiang Yun, Wang Yi-Tao, Wan Jian-Bo, Herbal medicines for the prevention of alcoholic liver disease: A review, 10.1016/j.jep.2012.09.044
  17. Huang Qing, Lu Guodong, Shen Han-Ming, Chung Maxey C.M., Ong Choon Nam, Anti-cancer properties of anthraquinones from rhubarb, 10.1002/med.20094
  18. Zhao Yan-Ling, Wang Jia-Bo, Zhou Guang-De, Shan Li-Mei, Xiao Xiao-He, Investigations of Free Anthraquinones from Rhubarb Against α-Naphthylisothiocyanate-induced Cholestatic Liver Injury in Rats, 10.1111/j.1742-7843.2009.00389.x
  19. Bindels Laure B., Neyrinck Audrey M., Salazar Nuria, Taminiau Bernard, Druart Céline, Muccioli Giulio G., François Emmanuelle, Blecker Christophe, Richel Aurore, Daube Georges, Mahillon Jacques, de los Reyes-Gavilán Clara G., Cani Patrice D., Delzenne Nathalie M., Non Digestible Oligosaccharides Modulate the Gut Microbiota to Control the Development of Leukemia and Associated Cachexia in Mice, 10.1371/journal.pone.0131009
  20. Neyrinck Audrey M., Possemiers Sam, Verstraete Willy, De Backer Fabienne, Cani Patrice D., Delzenne Nathalie M., Dietary modulation of clostridial cluster XIVa gut bacteria (Roseburia spp.) by chitin–glucan fiber improves host metabolic alterations induced by high-fat diet in mice, 10.1016/j.jnutbio.2010.10.008
  21. Esterbauer Hermann, Cheeseman Kevin H., [42] Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal, Oxygen Radicals in Biological Systems Part B: Oxygen Radicals and Antioxidants (1990) ISBN:9780121820879 p.407-421, 10.1016/0076-6879(90)86134-h
  22. Szabados Eszter, Fischer GaborM, Toth Kalman, Csete Bela, Nemeti Balazs, Trombitas Karoly, Habon Tamas, Endrei Dora, Sumegi Balazs, Role of reactive oxygen species and poly-ADP-ribose polymerase in the development of AZT-induced cardiomyopathy in rat, 10.1016/s0891-5849(98)00199-3
  23. De Conto C., Oevermann A., Burgener I. A., Doherr M. G., Blum J. W., Gastrointestinal tract mucosal histomorphometry and epithelial cell proliferation and apoptosis in neonatal and adult dogs, 10.2527/jas.2009-2511
  24. Kitazawa Hidefumi, Nishihara Tamao, Nambu Tadahiro, Nishizawa Hitoshi, Iwaki Masanori, Fukuhara Atsunori, Kitamura Toshio, Matsuda Morihiro, Shimomura Iichiro, Intectin, a Novel Small Intestine-specific Glycosylphosphatidylinositol-anchored Protein, Accelerates Apoptosis of Intestinal Epithelial Cells, 10.1074/jbc.m408047200
  25. Cliffe L. J., Accelerated Intestinal Epithelial Cell Turnover: A New Mechanism of Parasite Expulsion, 10.1126/science.1108661
  26. Szabo Gyongyi, Gut–Liver Axis in Alcoholic Liver Disease, 10.1053/j.gastro.2014.10.042
  27. Rao R. K., Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer, Alcohol (2008) ISBN:9781588299062 p.171-183, 10.1007/978-1-59745-242-7_13
  28. Wang Y., Liu Y., Sidhu A., Ma Z., McClain C., Feng W., Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury, 10.1152/ajpgi.00024.2012
  29. Turner Jerrold R., Intestinal mucosal barrier function in health and disease, 10.1038/nri2653
  30. Schnabl Bernd, Brenner David A., Interactions Between the Intestinal Microbiome and Liver Diseases, 10.1053/j.gastro.2014.01.020
  31. Adachi Yukito, Moore Laura E., Bradford Blair U., Gao Wenshi, Thurman Ronald G., Antibiotics prevent liver injury in rats following long-term exposure to ethanol, 10.1016/0016-5085(95)90027-6
  32. Ferrier Laurent, Bérard Florian, Debrauwer Laurent, Chabo Chantal, Langella Philippe, Buéno Lionel, Fioramonti Jean, Impairment of the Intestinal Barrier by Ethanol Involves Enteric Microflora and Mast Cell Activation in Rodents, 10.2353/ajpath.2006.050617
  33. Roberfroid Marcel, Gibson Glenn R., Hoyles Lesley, McCartney Anne L., Rastall Robert, Rowland Ian, Wolvers Danielle, Watzl Bernhard, Szajewska Hania, Stahl Bernd, Guarner Francisco, Respondek Frederique, Whelan Kevin, Coxam Veronique, Davicco Marie-Jeanne, Léotoing Laurent, Wittrant Yohann, Delzenne Nathalie M., Cani Patrice D., Neyrinck Audrey M., Meheust Agnes, Prebiotic effects: metabolic and health benefits, 10.1017/s0007114510003363
  34. Dueñas Montserrat, Muñoz-González Irene, Cueva Carolina, Jiménez-Girón Ana, Sánchez-Patán Fernando, Santos-Buelga Celestino, Moreno-Arribas M. Victoria, Bartolomé Begoña, A Survey of Modulation of Gut Microbiota by Dietary Polyphenols, 10.1155/2015/850902
  35. Anhê Fernando F., Varin Thibault V., Le Barz Mélanie, Desjardins Yves, Levy Emile, Roy Denis, Marette André, Gut Microbiota Dysbiosis in Obesity-Linked Metabolic Diseases and Prebiotic Potential of Polyphenol-Rich Extracts, 10.1007/s13679-015-0172-9
  36. Yan Arthur W., E. Fouts Derrick, Brandl Johannes, Stärkel Peter, Torralba Manolito, Schott Eckart, Tsukamoto Hide, E. Nelson Karen, A. Brenner David, Schnabl Bernd, Enteric dysbiosis associated with a mouse model of alcoholic liver disease, 10.1002/hep.24018
  37. Gallo Richard L., Hooper Lora V., Epithelial antimicrobial defence of the skin and intestine, 10.1038/nri3228
  38. Everard Amandine, Lazarevic Vladimir, Gaïa Nadia, Johansson Maria, Ståhlman Marcus, Backhed Fredrik, Delzenne Nathalie M, Schrenzel Jacques, François Patrice, Cani Patrice D, Microbiome of prebiotic-treated mice reveals novel targets involved in host response during obesity, 10.1038/ismej.2014.45
  39. Vereecke Lars, Beyaert Rudi, van Loo Geert, Enterocyte death and intestinal barrier maintenance in homeostasis and disease, 10.1016/j.molmed.2011.05.011
  40. Khan, Int. J. Chronic Dis., 2014, 789340 (2014)
  41. Hasper Ilona, Ventskovskiy Boris M., Rettenberger Reinhard, Heger Peter W., Riley David S., Kaszkin-Bettag Marietta, Long-term efficacy and safety of the special extract ERr 731 of Rheum rhaponticum in perimenopausal women with menopausal symptoms : , 10.1097/gme.0b013e3181806446
  42. Ding Yan, Zhao Lei, Mei Hong, Zhang Shu-Ling, Huang Zhi-Hua, Duan Yan-Ying, Ye Pian, Exploration of Emodin to treat alpha-naphthylisothiocyanate-induced cholestatic hepatitis via anti-inflammatory pathway, 10.1016/j.ejphar.2008.06.044
  43. Arosio Beatrice, Gagliano Nicoletta, Fusaro Lorena Maria Pia, Parmeggiani Luciano, Tagliabue Jacopo, Galetti Paola, De Castri Diego, Moscheni Claudia, Annoni Giorgio, Aloe-Emodin Quinone Pretreatment Reduces Acute Liver Injury Induced by Carbon Tetrachloride, 10.1034/j.1600-0773.2000.d01-79.x
  44. Jin Haiyan, Sakaida Isao, Tsuchiya Masako, Okita Kiwamu, Herbal medicine Rhei rhizome prevents liver fibrosis in rat liver cirrhosis induced by a choline-deficient L-amino acid-defined diet, 10.1016/j.lfs.2004.09.041
  45. Wang Jia-bo, Zhao Hai-ping, Zhao Yan-ling, Jin Cheng, Liu Dao-jian, Kong Wei-jun, Fang Fang, Zhang Lin, Wang Hong-juan, Xiao Xiao-he, Hepatotoxicity or Hepatoprotection? Pattern Recognition for the Paradoxical Effect of the Chinese Herb Rheum palmatum L. in Treating Rat Liver Injury, 10.1371/journal.pone.0024498
  46. Wang Jiabo, Zhao Yanling, Xiao Xiaohe, Li Huifang, Zhao Haiping, Zhang Ping, Jin Cheng, Assessment of the renal protection and hepatotoxicity of rhubarb extract in rats, 10.1016/j.jep.2009.04.018
  47. Dryden Gerald W., Deaciuc Ion, Arteel Gavin, McClain Craig J., Clinical implications of oxidative stress and antioxidant therapy, 10.1007/s11894-005-0024-y
  48. Morgan K, Production of a cytochrome P450 2E1 transgenic mouse and initial evaluation of alcoholic liver damage, 10.1053/jhep.2002.33720
  49. Kono Hiroshi, Rusyn Ivan, Yin Ming, Gäbele Erwin, Yamashina Shunhei, Dikalova Anna, Kadiiska Maria B., Connor Henry D., Mason Ronald P., Segal Brahm H., Bradford Blair U., Holland Steven M., Thurman Ronald G., NADPH oxidase–derived free radicals are key oxidants in alcohol-induced liver disease, 10.1172/jci9020
  50. Ceccarelli Sara, Toll-like receptor-mediated signaling cascade as a regulator of the inflammation network during alcoholic liver disease, 10.3748/wjg.v20.i44.16443
  51. Uesugi T, Toll-like receptor 4 is involved in the mechanism of early alcohol-induced liver injury in mice, 10.1053/jhep.2001.25350
  52. Beutler Bruce, Microbe sensing, positive feedback loops, and the pathogenesis of inflammatory diseases, 10.1111/j.1600-065x.2008.00733.x
  53. Everard A., Belzer C., Geurts L., Ouwerkerk J. P., Druart C., Bindels L. B., Guiot Y., Derrien M., Muccioli G. G., Delzenne N. M., de Vos W. M., Cani P. D., Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity, 10.1073/pnas.1219451110
  54. Everard Amandine, Lazarevic Vladimir, Derrien Muriel, Girard Myriam, Muccioli Giulio G., Neyrinck Audrey M., Possemiers Sam, Van Holle Ann, François Patrice, de Vos Willem M., Delzenne Nathalie M., Schrenzel Jacques, Cani Patrice D., Responses of Gut Microbiota and Glucose and Lipid Metabolism to Prebiotics in Genetic Obese and Diet-Induced Leptin-Resistant Mice, 10.2337/db11-0227
  55. Chang Chih-Jung, Lin Chuan-Sheng, Lu Chia-Chen, Martel Jan, Ko Yun-Fei, Ojcius David M., Tseng Shun-Fu, Wu Tsung-Ru, Chen Yi-Yuan Margaret, Young John D., Lai Hsin-Chih, Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota, 10.1038/ncomms8489
  56. Delzenne Nathalie M., Bindels Laure B., Gut microbiota: Ganoderma lucidum, a new prebiotic agent to treat obesity?, 10.1038/nrgastro.2015.137
Bibliographic reference Neyrinck, Audrey M. ; Etxeberria, Usune ; Taminiau, Bernard ; Daube, Georges ; Van Hul, Matthias ; et. al. Rhubarb extract prevents hepatic inflammation induced by acute alcohol intake, an effect related to the modulation of the gut microbiota.. In: Molecular Nutrition & Food Research, Vol. 61, no. 1, p. 1500899 [1-12] (2017)
Permanent URL http://hdl.handle.net/2078.1/175106