User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Contribution of oxygenation to BOLD contrast in exercising muscle

Primary tabs

Jordan, Bénédicte [UCL] Kimpalou, José Zéfu [UCL] Beghein, Nelson [UCL] Dessy, Chantal [UCL] Feron, Olivier [UCL] Gallez, Bernard [UCL]

The potential physiological and therapeutic applications of functional MRI (fMRI) in skeletal muscle will depend on our ability to identify factors that may contribute to fluctuations in the BOLD signal. Until now, interpretations of signal changes in fMRI studies of muscle have mostly relied on the increase in muscle T2 associated with osmotically driven fluid shifts. However, recent studies have documented increases in BOLD signal intensity (SI) after single contractions, coinciding with increases in muscle hemoglobin saturation. In this study, the factors that contribute to variations in the intensity of the BOLD signal in exercising muscle are further addressed. For this purpose, BOLD imaging was performed during and after a moderate electrical stimulation was applied to the sciatic nerve in mice. In addition, oxygen pressure (pO2), blood flow, and skeletal muscle T2 (fast and slow components: T2 and T'2, respectively) were monitored. A comparison between mice lacking eNOS (eNOS-/- mice) and their wild-type (WT) littermates was performed. In WT mice, the BOLD SI, as well as muscle oxygenation and T'2, were significantly increased for a prolonged time in response to this moderate exercise protocol. Blood flow immediately dropped after the electrical stimulation was stopped. In eNOS-/- mice, the high BOLD SI did not persist after the exercise protocol ended. This finding correlates well with the evolution of muscle oxygenation, which progressively decreases after stimulation in eNOS-/- mice. However, T'2 remained high for a prolonged time after stimulation. We therefore concluded that the maintenance of BOLD SI in moderately exercising skeletal muscle depends mainly on changes in pO2, rather than on blood flow or T2 effects.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2004
Language Anglais
Journal information "Magnetic Resonance in Medicine" - Vol. 52, no. 2, p. 391-396 (2004)
Peer reviewed yes
Publisher JohnWiley & Sons, Inc. ((United States) Hoboken)
issn 0740-3194
e-issn 1522-2594
Publication status Publié
Affiliations UCL - MD/FARM - Ecole de pharmacie
UCL - MD/MINT - Département de médecine interne
UCL - MD/RAIM - Département de radiologie et d'imagerie médicale
UCL - (SLuc) Service de médecine nucléaire
MESH Subject Analysis of Variance ; Animals ; Magnetic Resonance Imaging - methods ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Muscle, Skeletal - metabolism ; Oxygen - metabolism ; Physical Conditioning, Animal - physiology
Links
  1. Ogawa, Proc Natl Acad Sci USA, 87, 9868 (1990)
  2. Karczmar, NMR Biomed, 7, 3 (1994)
  3. Griffiths, Int J Radiat Oncol Biol Phys, 39, 697 (1997)
  4. Al-Hallaq, Int J Radiat Oncol Biol Phys, 41, 151 (1998)
  5. Jordan, Int J Radiat Oncol Biol Phys, 48, 565 (2000)
  6. Baudelet, Magn Reson Med, 48, 980 (2002)
  7. A study of the MR signal produced by ischemia in human subjects. In: Proceedings of the 4th Annual Meeting of ISMRM, New York, 1996. p 1773.
  8. Donahue, J Magn Reson Imaging, 8, 1106 (1998)
  9. Lebon, Magn Reson Med, 40, 551 (1998)
  10. Time resolved observation of BOLD effect in muscle during isometric exercise. In: Proceedings of the 8th Annual Meeting of ISMRM, Denver, 2000. p 122.
  11. BOLD MRI and NIRS detection of transient hyperthermia after single skeletal muscle contractions. In: Proceedings of the 9th Annual Meeting of ISMRM, Glasgow, Scotland, 2001. p 135.
  12. Field strength dependence of transient BOLD signal changes in skeletal muscle after single contractions. In: Proceedings of the 11th Annual Meeting of ISMRM, Toronto, Canada, 2003. p 1521.
  13. Meyer, Exer Sport Sci Rev, 28, 89 (2000)
  14. Stainsby, Magn Reson Med, 45, 662 (2001)
  15. Prior, J Appl Physiol, 87, 2107 (1999)
  16. Prior, J Appl Physiol, 90, 615 (2001)
  17. Gambarota, Magn Reson Med, 46, 592 (2001)
  18. van Zijl, Nat Med, 4, 159 (1998)
  19. Simultaneous measurement of gradient-echo 1H MR images and pO2 using a fiber-optic oxygen sensor in rodent tumours and their response to carbogen breathing. In: Proceedings of the 6th Annual Meeting of ISMRM, Sydney, Australia, 1998. p 1995.
  20. Brown, Acta Physiol Scand, 168, 667 (2000)
  21. Reid, Acta Physiol Scand, 162, 401 (1998)
  22. Goudemant, Neuromuscul Disord, 8, 371 (1998)
  23. Gallez, Magn Reson Med, 42, 627 (1999)
  24. Jordan, Magn Reson Mater Phys Med Biol, 7, 121 (1998)
  25. Collingridge, Radiat Res, 147, 329 (1997)
  26. Griffiths J R, Robinson S P, The OxyLite: a fibre-optic oxygen sensor., 10.1259/bjr.72.859.10624317
  27. Andersen P, Saltin B, Maximal perfusion of skeletal muscle in man., 10.1113/jphysiol.1985.sp015794
  28. Radegran, Am J Physiol, 274, h314 (1998)
  29. Radegran, Acta Physiol Scand, 168, 575 (2000)
  30. Delp, Acta Physiol Scand, 162, 411 (1998)
  31. Saltin, Acta Physiol Scand, 162, 421 (1998)
  32. Boushel, Acta Physiol Scand, 168, 615 (2000)
  33. Richardson, J Appl Physiol, 91, 2679 (2001)
  34. Shen W., Hintze T. H., Wolin M. S., Nitric Oxide : An Important Signaling Mechanism Between Vascular Endothelium and Parenchymal Cells in the Regulation of Oxygen Consumption, 10.1161/01.cir.92.12.3505
  35. Wolin M. S., Hintze T. H., Shen W., Mohazzab-H. K. M., Xie Y.-W., Involvement of reactive oxygen and nitrogen species in signalling mechanisms that control tissue respiration in muscle, 10.1042/bst0250934
  36. Moncada, Nat Rev Mol Cell Biol, 3, 214 (2002)
Bibliographic reference Jordan, Bénédicte ; Kimpalou, José Zéfu ; Beghein, Nelson ; Dessy, Chantal ; Feron, Olivier ; et. al. Contribution of oxygenation to BOLD contrast in exercising muscle. In: Magnetic Resonance in Medicine, Vol. 52, no. 2, p. 391-396 (2004)
Permanent URL http://hdl.handle.net/2078.1/9905