User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Freeze-dried irradiated bone brittleness improves compactness in an impaction bone grafting model.

Primary tabs

Cornu, Olivier [UCL] Libouton, Xavier [UCL] Naets, Bénédicte [UCL] Godts, Bernard [UCL] Van Tomme, John [Department of Civil Engineering, Royal Military Academy, Brussels, Belgium] Delloye, Christian [UCL] Banse, Xavier [UCL]

BACKGROUND: Defatted bone chips with or without freeze-drying and irradiation have mechanical advantages as compared to fresh-frozen controls in in vitro models of impaction. These improved results have been ascribed to replacement of viscous bone marrow by saline and embrittlement of the freeze-dried bone by irradiation. MATERIAL AND METHODS: To determine which of these hypotheses is correct, we compared the development of stiffness and compactness of morselized bone graft that had been: 1) fat-reduced with saline, and 2) fresh-frozen, solvent-detergent defatted, 3) freeze-dried irradiated and 4) not irradiated. We used 12 osteoarthrotic femoral heads to prepare these four batches of morselized bone, and impacted 18 samples from each batch in a cylinder. The frozen bone grafts were tested after thawing at room temperature for 2 hours and the freeze-dried grafts were tested after 30 minutes of rehydration. We monitored the development of compactness and stiffness of the material during impaction. RESULTS: The stiffness of the freeze-dried irradiated bone was greater than that of the other three series after 10, 50 and 150 impactions. The freeze-dried bone chips that were not irradiated and the chips defatted with saline alone were less stiff than the fresh-frozen control after 150 impactions. INTERPRETATION: The brittleness of freeze-dried irradiated bone, caused by loss of the capacity to absorb energy in a plastic way, increases the compactness and stiffness of the morselized grafts. Washing bone with saline alone or treating bone with solvent-detergent but no irradiation had no similar mechanical advantage and the bone did not impact better than fresh-frozen undefatted bone in our model.

Document type Article de périodique (Journal article) – Journal Article
Access type Accès restreint
Publication date 2004
Journal information "Acta orthopaedica Scandinavica" - Vol. 75, no. 3, p. 309-314 (2004)
Peer reviewed yes
Publisher Taylor & Francis ((United Kingdom) Basingstoke)
issn 0001-6470
Publication status Publié
Affiliations UCL - MD/CHIR - Département de chirurgie
UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur
UCL - (SLuc) Centre du cancer
UCL - (SLuc) Centre de thérapie tissulaire et cellulaire
MESH Subject Aged ; Aged, 80 and over ; Bone Transplantation - methods ; Female ; Femur Head - radiation effects - transplantation ; Freeze Drying ; Humans ; Male ; Middle Aged ; Tissue and Organ Harvesting
Links
  1. Bavadekar A, Stiffness and compactness of morselized grafts during impaction: an in vitro study with human femoral heads. Acta Orthop Scand, 75, 470 (2001)
  2. Carter D R, Hayes W C., J Bone Joint Surg (Am), 59, 954 (1977)
  3. Cornu, Effect of freeze-drying and gamma irradiation on the mechanical properties of human cancellous bone. J Orthop Res, 18, 426 (2000)
  4. Cornu, Impaction bone grafting with freeze-dried irra-diated bone. Part I. Femoral implant stability. Acta Orthop Scand, 74, 547 (2003)
  5. Cornu, Impaction bone grafting with freeze-dried irradiated bone. Part II. Changes in stiffness and compact-ness of morselized grafts. Acta Orthop Scand, 74, 553 (2003)
  6. de Roeck Nicholas J., Drabu Khalid J., Impaction bone grafting using freeze-dried allograft in revision hip arthroplasty, 10.1054/arth.2001.20250
  7. Hostner J, Impaction technique and graft treatment in revisions of the femoral component: laboratory studies and clinical validation. J Arthroplasty, 16, 76 (2001)
  8. Karrholm J, Evaluation with radiostereometry and dual-energy X-ray absorptiometry. J Bone Joint Surg (Br), 81, 135 (1999)
  9. Linder Lars, Cancellous impaction grafting in the human femur: Histological and radiographic observations in 6 autopsy femurs and 8 biopsies, 10.1080/000164700317362154
  10. Mazhar T A, Aydin M, Atilla B, Caner B., Arch Orthop Trauma Surg, 120, 416 (2001)
  11. Tagil M, Clin Orthop, 352, 231 (1998)
  12. Thien Truike M., Welten Marianne L.M., Verdonschot Nico, Buma Pieter, Yong Pu, Schreurs B.Willem, Acetabular revision with impacted freeze-dried cancellous bone chips and a cemented cup, 10.1054/arth.2001.23582
  13. Thoren K, Lipid-extracted bank bone. Bone conductive and mechanical properties. Chin Orthop, 311, 232 (1995)
  14. Ullmark G., Bigger size and defatting of bone chips will increase cup stability, 10.1007/s004029900122
Bibliographic reference Cornu, Olivier ; Libouton, Xavier ; Naets, Bénédicte ; Godts, Bernard ; Van Tomme, John ; et. al. Freeze-dried irradiated bone brittleness improves compactness in an impaction bone grafting model.. In: Acta orthopaedica Scandinavica, Vol. 75, no. 3, p. 309-314 (2004)
Permanent URL http://hdl.handle.net/2078.1/9899